NDLI: miR-145 targets the SOX11 3'UTR to suppress endometrial cancer growth.

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miR-145 targets the SOX11 3'UTR to suppress endometrial cancer growth.

Content Provider	Semantic Scholar
Author	Chang, Lei Yuan, Zhongfu Shi, Huirong Bian, Yangyang Guo, Ruixia
Copyright Year	2017
Abstract	To explore the functions of SOX (Sex determining Region Y-related HMG-box) family genes in endometrial cancer (EC) and determine the influence of miR-145/SOX11 on EC cell functions. The relationship between miR-145 and SOX11 was confirmed using TargetScan, miRNA databases and dual-luciferase reporter gene assays. The expression of SOX11 mRNA in tissue specimens was examined using RT-qPCR, while SOX11 protein expression in tissues and cell lines were detected through immunohistochemistry (IHC) and western blotting. After transfection using Lipofectamine 2000, the proliferation, migration, invasion and apoptosis of ECC-1 and HEC-1-A cells were assessed through colony formation, transwell and flow cytometry assays. The correlation of SOX11 expression with the prognosis outcomes of patients was analyzed using Kaplan-Meier analysis and the log-rank test. SOX11 showed high expression in EC, which is negatively correlated with a poor prognostic outcome of EC patients. The expression of miR-145 was lower in EC tissues than in adjacent tissues. MiR-145 significantly reduced the expression of SOX11. In ECC-1 cells, miR-145 suppressed the propagation, migration, and invasion of cells and promoted cell apoptosis. MiR-145 also inhibited the proliferation, migration, and invasion of HEC-1-A cells and facilitated cell apoptosis by inhibiting SOX11. MiR-145 targeted site 3 (3615) of the SOX11 3'UTR to affect the expression of SOX11. MiR-145 and its target gene SOX11 could serve as diagnostic markers for EC. MiR-145 targets the SOX11 3'UTR to inhibit its expression and suppress the propagation and metastasis of EC cells.
Starting Page	2305
Ending Page	2317
Page Count	13
File Format	PDF HTM / HTML
PubMed reference number	29218252
Journal	Medline
Volume Number	7
Issue Number	11
Alternate Webpage(s)	http://ajcr.us/files/ajcr0064398.pdf
Journal	American journal of cancer research
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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