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Astrocytes derived from glial-restricted precursors promote spinal cord repair
| Content Provider | Semantic Scholar |
|---|---|
| Author | Huang, Carol Proschel, Christoph Noble, Mark Mayer-Proschel, Margot |
| Copyright Year | 2006 |
| Abstract | BACKGROUND Transplantation of embryonic stem or neural progenitor cells is an attractive strategy for repair of the injured central nervous system. Transplantation of these cells alone to acute spinal cord injuries has not, however, resulted in robust axon regeneration beyond the sites of injury. This may be due to progenitors differentiating to cell types that support axon growth poorly and/or their inability to modify the inhibitory environment of adult central nervous system (CNS) injuries. We reasoned therefore that pre-differentiation of embryonic neural precursors to astrocytes, which are thought to support axon growth in the injured immature CNS, would be more beneficial for CNS repair. RESULTS Transplantation of astrocytes derived from embryonic glial-restricted precursors (GRPs) promoted robust axon growth and restoration of locomotor function after acute transection injuries of the adult rat spinal cord. Transplantation of GRP-derived astrocytes (GDAs) into dorsal column injuries promoted growth of over 60% of ascending dorsal column axons into the centers of the lesions, with 66% of these axons extending beyond the injury sites. Grid-walk analysis of GDA-transplanted rats with rubrospinal tract injuries revealed significant improvements in locomotor function. GDA transplantation also induced a striking realignment of injured tissue, suppressed initial scarring and rescued axotomized CNS neurons with cut axons from atrophy. In sharp contrast, undifferentiated GRPs failed to suppress scar formation or support axon growth and locomotor recovery. CONCLUSION Pre-differentiation of glial precursors into GDAs before transplantation into spinal cord injuries leads to significantly improved outcomes over precursor cell transplantation, providing both a novel strategy and a highly effective new cell type for repairing CNS injuries. |
| Starting Page | 7 |
| Ending Page | 7 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://download-redirector.springer.com/redirect?contentType=pdf&ddsId=art:10.1186/jbiol35&originUrl=http://jbiol.biomedcentral.com/article/10.1186/jbiol35 |
| Alternate Webpage(s) | http://jbiol.com/content/pdf/jbiol35.pdf |
| Alternate Webpage(s) | https://static-content.springer.com/esm/art:10.1186%2Fjbiol35/MediaObjects/13061_2005_72_MOESM2_ESM.pdf |
| PubMed reference number | 16643674 |
| Volume Number | 5 |
| Journal | Journal of biology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Atrophic CNS disorder Cell Transplantation Cervical Undifferentiated Carcinoma Cicatrix Cicatrization Dorsal funiculus GDA gene Hematopoietic stem cells Intersex Nervous system structure Neuroglia Spinal Cord Injuries Spinal Cord Regeneration Structure of rubrospinal tract Traumatic injury axon regeneration cell type central nervous system tumor, pediatric |
| Content Type | Text |
| Resource Type | Article |
