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Crystallization of MoFe Protein ( DnifH Av1) from a nifH Deleted Strain of Azotobacter vinelandii
| Content Provider | Semantic Scholar |
|---|---|
| Author | Shao-Min, Bian Jian-Feng, Zhao Yu-Bing, L. Ying, Zhao Hui-Na, Zhou Yao-Ping, Wang Ju-Fu, Huang |
| Copyright Year | 2004 |
| Abstract | A FeMoco-deficient ∆nifH Av1 was partially purified from a nifH deleted mutant DJ54 of Azotobacter vinelandii Lipmann grown in NH3-limited medium. By using the same purification method, ∆nifE Av1 and NifBAv1 were obtained from DJ35 and UW45, respectively. The latter two proteins were obviously much purer than ∆nifH Av1. Under a suitable condition for crystallization, dark brown short rhombohedron crystals could be obtained from the three proteins. Like NifBAv1, the time for formation of ∆nifH Av1 crystal was longer than that of ∆nifE Av1. But the optimal concentrations of precipitant and buffer for crystallization of ∆nifH Av1 were similar to those of ∆nifE Av1. SDS-PAGE analysis showed that the crystalline ∆nifH Av1 was similar in the composition to OP Av1. It indicates that the crystal formed in ∆nifH Av1 solution could be the protein crystal. |
| Starting Page | 58 |
| Ending Page | 62 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Volume Number | 46 |
| Alternate Webpage(s) | http://www.jipb.net/pubsoft/content/2/3511/x030024.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
