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Helicobacter pylori
| Content Provider | Semantic Scholar |
|---|---|
| Author | Covacci, Antonello Rappuoli, Rino |
| Copyright Year | 2003 |
| Abstract | The race to sequence the human genome generated a global emotional wave which escaped the scientific community and involved the media, the politicians, the economic world, and the general public. The sequencing of several bacterial genomes also generated a high interest. A few years after publication of the genomes we are back at the bench, performing ad hoc experiments and asking ourselves what the genomic wave meant and how it changed our lives. The genome of Helicobacter pylori , the bacterium which causes peptic ulcer and gastric cancer, was published back in 1997 (1), and it is very old in genomic terms (H. pylori was the fourth bacterial genome to be published after the one of Haemophilus influenzae , Mycoplasma genitalium , and Methanococcus jannaschii). H. pylori was also the first bacterium for which the genomes of two different strains were determined (2), and the first one for which maps of pro-tein–protein interactions were published (3). In spite of this, our knowledge is still limited and pregenomic experiments are still needed to unravel the secrets of how this bacterium causes disease. A paper describing the first application of signature tagged mutagenesis (STM) to identify virulence factors of H. pylori , published in this issue (4), provides us with an opportunity to think about the biology in the postgenomic era, the role of the pregenomic techniques in general, and also what the new findings mean for H. pylori. New Insights for H. pylori Colonization and Virulence. Kavermann et al. collected 960 mutants of Helicobacter py-lori , one of the largest collections described in literature, and tested them for colonization into a suitable animal model (Mongolian gerbils) by using STM to identify genes essential for survival within the animal host. In the original STM developed for Salmonella (5), a library of mutants, obtained by transposon mutagenesis and each tagged by a unique sequence, were used in pools to infect an animal model. The mutants which did not survive the infection in vivo were then identified by the absence of the tag. Given the difficulty of H. pylori genetic manipulation, a library large enough to represent the entire genome could not be isolated; however, although incomplete, the one obtained allowed finding of some of the known virulence factors and also of some new ones. Among the known factors, most of the genes involved in flagella biosynthesis and four of the genes involved in … |
| Starting Page | 807 |
| Ending Page | 811 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 12668641v1 |
| Volume Number | 197 |
| Journal | The Journal of experimental medicine |
| Alternate Webpage(s) | https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0b/81/20030063.PMC2193897.pdf |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Biopolymer Sequencing Flagella Genome Genome, Bacterial Gerbils Helicobacter pylori Ab:ACnc:Pt:Ser:Qn Helicobacter pylori:PrThr:Pt:Gast fld:Ord:Organism specific culture Hemophilus ducreyi Influenza Meriones unguiculatus Mycoplasma genitalium Partial Peptic Ulcer Salmonella Scientific Publication Stomach Carcinoma Tracer Virulence genetic manipulation mutant |
| Content Type | Text |
| Resource Type | Article |
