Dominant inhibitory adipocyte-specific secretory factor (ADSF)/resistin enhances adipogenesis and improves insulin sensitivity.

Content Provider	Semantic Scholar
Author	Kim, Kee-Hong Zhao, Ling Moon, Yangsoo Kang, Chulho Sul, Hei Sook
Copyright Year	2004
Abstract	Adipocyte-specific secretory factor (ADSF)/resistin is a small cysteine-rich protein secreted from adipose tissue that belongs to a gene family found in inflammatory zone (FIZZ) or found in resistin-like molecule (RELM). ADSF has been implicated in modulating adipogenesis and insulin resistance. To examine the long-term function of ADSF in adipogenesis and glucose homeostasis, we constructed an expression vector for a dominant inhibitory form of ADSF by fusing it to the human IgGgamma constant region (hFc). ADSF-hFc not only homodimerizes but heterooligomerizes with ADSF/resistin and prevents ADSF/resistin inhibition of adipocyte differentiation of 3T3-L1 cells in a dominant negative manner. Transgenic mice overexpressing ADSF-hFc in adipose tissue show increased adiposity with elevated expression of adipocyte markers as well as enlarged adipocyte size. This finding clearly demonstrates in vivo the inhibitory role of ADSF in adipogenesis. ADSF-hFc transgenic mice with impaired ADSF function exhibit improved glucose tolerance and insulin sensitivity either on chow or high-fat diets. Because of the enhanced adipocyte differentiation, the ADSF-hFc transgenic mice show increased expression of leptin and adiponectin in adipose tissue. The elevated circulating levels for these adipocyte-derived hormones with decreased plasma triglyceride and free fatty acid levels may account for the improved glucose and insulin tolerance in these transgenic mice.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.pnas.org/content/101/17/6780.full.pdf
PubMed reference number	15090646v1
Volume Number	101
Issue Number	17
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Subject Keyword	Adipocytes Adipogenesis Adiponectin Adipose tissue CSRP1 gene Cell secretion Diet, High-Fat Dominant-Negative Mutation Enlargement procedure Insulin Resistance Nonesterified Fatty Acids Obesity RETN gene RETN wt Allele Triglycerides chow chow fat cell differentiation glucose homeostasis glucose tolerance insulin, isophane leptin resistin
Content Type	Text
Resource Type	Article