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Quantitative analysis of metabolic pathways in Catharanthus roseus hairy roots metabolically engineered for terpenoid indole alkaloid overproduction
| Content Provider | Semantic Scholar |
|---|---|
| Author | Sander, Guy W. |
| Copyright Year | 2009 |
| Abstract | The important anticancer pharmaceuticals, vinblastine and vincristine, are produc ed by Catharanthus roseus. Given their cytotoxicity, these valuable alkaloids are produced in very small quantities within the aerial parts of the plant. The high cost of isola ting the drugs has led to research efforts to increase the alkaloid content of C. r seus cell cultures, tissue cultures, and seedlings. The metabolic engineering of C. roseus strives to overcome the strict regulation of the biosynthetic pathways. Seedlings of C. roseus were elicited with methyl jasmonate (MeJA) to induce expression of octadecanoid-responsive Catharanthus AP2-domain 3 (ORCA3), a transcription regulator of several biosynthetic genes. ORCA3 exhibited increases up to 25fold observed 0.5 h after MeJA treatment with the transcript levels of biosyntheti c genes following with variable timing. The amounts of certain terpenoid indole alkaloid (TI A) metabolites, including the important vinblastine precursors, catharanthine and vindo line, were increased significantly. Three hairy root cultures of C. roseus were investigated. The ASAB-1 line expressing a feedback-resistant anthranilate synthase (AS) α subunit from Arabidopsis under the control of a glucocorticoid-inducible promoter and an AS β subunit from Arabidopsis under the control of the constitutive CaMV 35S promoter, the EHIDXS-4-1 line express ing 1-deoxy-D-xylulose 5-phosphate synthase (DXS) under the control of a glucocor ti idinducible promoter, and the EHIT16H-34-1 line tabersonine 16-hydroxylase (T16H) under the control of a glucocorticoid-inducible promoter. These lines were used to investi gat the regulatory nature of the biosynthetic network by quantifying the effect of l ight-adaptation, biosynthetic enzyme overexpression, and the combination of these two factors on the |
| File Format | PDF HTM / HTML |
| DOI | 10.31274/etd-180810-855 |
| Alternate Webpage(s) | https://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1874&context=etd&httpsredir=1&referer= |
| Alternate Webpage(s) | http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1874&context=etd |
| Alternate Webpage(s) | https://doi.org/10.31274/etd-180810-855 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
