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MEF-2 function is modified by a novel co-repressor, MITR.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Sparrow, Duncan B. Miska, Eric A. Langley, Emma Reynaud-Deonauth, Sorogini Kotecha, Surendra Towers, Norma Spohr, Georges Kouzarides, Tony Mohun, Timothy |
| Copyright Year | 1999 |
| Abstract | The MEF-2 proteins are a family of transcriptional activators that have been detected in a wide variety of cell types. In skeletal muscle cells, MEF-2 proteins interact with members of the MyoD family of transcriptional activators to synergistically activate gene expression. Similar interactions with tissue or lineage-specific cofactors may also underlie MEF-2 function in other cell types. In order to screen for such cofactors, we have used a transcriptionally inactive mutant of Xenopus MEF2D in a yeast two-hybrid screen. This approach has identified a novel protein expressed in the early embryo that binds to XMEF2D and XMEF2A. The MEF-2 interacting transcription repressor (MITR) protein binds to the N-terminal MADS/MEF-2 region of the MEF-2 proteins but does not bind to the related Xenopus MADS protein serum response factor. In the early embryo, MITR expression commences at the neurula stage within the mature somites and is subsequently restricted to the myotomal muscle. In functional assays, MITR negatively regulates MEF-2-dependent transcription and we show that this repression is mediated by direct binding of MITR to the histone deacetylase HDAC1. Thus, we propose that MITR acts as a co-repressor, recruiting a specific deacetylase to downregulate MEF-2 activity. |
| Starting Page | 35 |
| Ending Page | 41 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://embojnl.embopress.org/content/embojnl/18/18/5085.full.pdf |
| PubMed reference number | 10487760v1 |
| Volume Number | 18 |
| Issue Number | 18 |
| Journal | The EMBO journal |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Activation action Gene Expression Histone Deacetylase Histones MEF2D gene Muscle Cells Neurula Physical Inactivity Repression, Psychology Serum Proteins Somites Transcription Repressor/Corepressor Transcription, Genetic cell type chemical cofactor |
| Content Type | Text |
| Resource Type | Article |
