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Naked-eye detection as a universal approach to lower the limit of detection of enzyme-linked immunoassays
| Content Provider | Semantic Scholar |
|---|---|
| Author | O'Connor, E. Fitzgerald Paterson, Sureyya Rica, Roberto De La |
| Copyright Year | 2016 |
| Abstract | Colorimetric biosensors for the detection of analytes with the naked eye are required in environmental monitoring, point-of-care diagnostics, and analyses in resources constrained settings, where detection instruments may not be available. However, instrument-based detection methods are usually more adequate for detecting small variations in the signal compared to naked-eye detection schemes, and consequently the limit of detection of the latter is usually higher than the former. Here, we demonstrate that the limit of detection of colorimetric enzyme-linked immunoassays can be decreased several orders of magnitude when using naked-eye detection instead of a spectrophotometer for detecting the signal. The key step to lower the limit of detection is adding a small volume of chromogenic substrate during the signal generation step. This generates highly colored solutions that can be easily visualized with the naked eye and recorded with the camera of a mobile phone. The proposed method does not require expensive equipment or complex protocols to enhance the signal, and therefore it is a universal approach to lower the limit of detection of colorimetric enzyme-linked immunoassays. |
| Starting Page | 3389 |
| Ending Page | 3393 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s00216-016-9453-8 |
| PubMed reference number | 26970749 |
| Journal | Medline |
| Volume Number | 408 |
| Alternate Webpage(s) | https://strathprints.strath.ac.uk/55787/1/OConnor_Paterson_de_la_Rica_ABC2016_Naked_eye_detection_as_a_universal_approach_to_lower_the_limit.pdf |
| Alternate Webpage(s) | https://pureportal.strath.ac.uk/files-asset/46624904/OConnor_Paterson_de_la_Rica_ABC2016_Naked_eye_detection_as_a_universal_approach_to_lower_the_limit.pdf |
| Alternate Webpage(s) | https://doi.org/10.1007/s00216-016-9453-8 |
| Journal | Analytical and Bioanalytical Chemistry |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
