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Construction and Expression of a Single Chain Antibody Mimicing Human Ovarian Cancer Antigen CA 125
| Content Provider | Semantic Scholar |
|---|---|
| Author | Li, Aidong Li, Zheng Wang, Yinghong Zhang, Yongming |
| Copyright Year | 2006 |
| Abstract | One concept for immune therapy of cancer involves induction of antigen mimic antibodies to trigger the immune response against tumor cells. Anti-idiotypic antibodies directed against the antigen-binding site of antibodies specific for tumor antigen may functionally and even structurally mimic antigen and induce anti-anti-idiotypic immune response. Monoclonal antibody WJ02 is one of such anti-idiotypic antibodies, which contains internal image of CA125. In order to improve the immunospecificity of mAb WJ02, we constructed a single chain of mAb WJ02 in Vl-linker-Vh orientation. The scFv-WJ02 could be expressed and secreted in the recombinant Pichia pastoris system. The secreted scFv protein with a molecular weight of 30 kD retained the biological activity of mAb WJ02, which was proved by a direct binding assay and inhibition experiment. Our results indicated that the scFv-WJ02 could be used as a possible tool for idiotypic therapy against ovarian cancer, which might enhance the possibility of eliminating nonspecific responses induced by mAb WJ02. Cellular & Molecular Immunology. 2006; 3(1):59-62. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.cmi.ustc.edu.cn/3/1/59.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
