Depigmented and polymerised house dust mite allergoid: allergen content, induction of IgG4 and clinical response.

Content Provider	Semantic Scholar
Author	Gallego, María Teresa González Iraola, Víctor Himly, Martin Robinson, D. Badiola, Carlos M. García-Robaina, José-Carlos Richter, Klaus W. Carnés, Jerónimo
Copyright Year	2010
Abstract	BACKGROUND Polymerised allergenic extracts (allergoids) are commonly used in allergen immunotherapy. Clinical efficacy and safety of these extracts have been demonstrated. Recently, allergen sequences have been identified by mass spectrometry in depigmented and polymerised (Dpg-Pol) extracts. The objectives of this study were to investigate the presence of allergens in Dpg-Pol extracts of house dust mite and to analyze the immunological changes induced by these extracts in asthmatic patients enrolled in a double-blind, placebo-controlled study. METHODS Dpg-Pol extracts were manufactured and vaccines with a composition of 50% Dermatophagoides pteronyssinus and 50% D. farinae (100 HEPL/ml) were prepared. Allergen composition was analyzed by mass spectrometry. Patients with asthma and rhinoconjunctivitis were treated in a 1-year, double-blind, placebo-controlled, parallel-group study with 6 up-dosing and monthly maintenance injections. Specific IgE and IgG4 titres to D. pteronyssinus, Der p 1 and Der p 2 were measured in patients' sera using the CAP system and direct ELISA experiments. RESULTS Sequences from the major allergens Der p 1 and Der p 2 and from other allergens were identified in native and Dpg-Pol extracts. There was a statistically significant increase in specific IgG4, a decrease in the ratio of IgE/IgG4 to D. pteronyssinus and a significant increase in specific IgG4 to Der p 1 and Der p 2 in the patients allotted to active treatment. CONCLUSIONS The detection of allergen sequences suggests preservation of major and minor allergens in Dpg-Pol allergoids from house dust mites. Efficacy in asthma treatment and the increase in specific IgG4 seem to be associated with the presence of major allergens in Dpg-Pol allergen extracts.
Starting Page	669
Ending Page	679
Page Count	11
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.karger.com/Article/Pdf/301580
Alternate Webpage(s)	http://www.karger.com/Article/Pdf/301580
PubMed reference number	20357486v1
Alternate Webpage(s)	https://doi.org/10.1159/000301580
DOI	10.1159/000301580
Journal	International archives of allergy and immunology
Volume Number	153
Issue Number	1
Language	English
Access Restriction	Open
Subject Keyword	Allergens Asthma Biologic Preservation Dermatophagoides farinae Dermatophagoides pteronyssinus Enzyme-Linked Immunosorbent Assay House Dust Immunoglobulin G Subclass 4 Measurement Immunoglobulin IgG4 Immunotherapy Mite Infestations Mites Patients Pyroglyphidae Spectrometry
Content Type	Text
Resource Type	Article