Loading...
Please wait, while we are loading the content...
Similar Documents
Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kim, Jaeug Kim, Myungji Hwang, Dong Gyu Kim, Song Cheol Jang, Jinah |
| Copyright Year | 2019 |
| Abstract | The transplantation of pancreatic islets is a promising treatment for patients who suffer from type 1 diabetes accompanied by hypoglycemia and secondary complications. However, islet transplantation still has several limitations such as the low viability of transplanted islets due to poor islet engraftment and hostile environments. In addition, the insulin-producing cells differentiated from human pluripotent stem cells have limited ability to secrete sufficient hormones that can regulate the blood glucose level; therefore, improving the maturation by culturing cells with proper microenvironmental cues is strongly required. In this article, we elucidate protocols for preparing a pancreatic tissue-derived decellularized extracellular matrix (pdECM) bioink to provide a beneficial microenvironment that can increase glucose sensitivity of pancreatic islets, followed by describing the processes for generating 3D pancreatic tissue constructs using a microextrusion-based bioprinting technique. |
| File Format | PDF HTM / HTML |
| DOI | 10.3791/60434 |
| PubMed reference number | 31885383 |
| Journal | Medline |
| Volume Number | 154 |
| Journal | Journal of visualized experiments : JoVE |
| Alternate Webpage(s) | https://www.jove.com/pdf-materials/60434/jove-materials-60434-pancreatic-tissue-derived-extracellular-matrix-bioink-for-printing-3d |
| Alternate Webpage(s) | https://doi.org/10.3791/60434 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
