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Effects of Eugenol and MS-222 Anaesthesia on Siberian Sturgeon Acipenser baerii Brandt
| Content Provider | Semantic Scholar |
|---|---|
| Author | Gomułka, Piotr Własow, Teresa Velisek, Josef Svobodová, Zdenka Chmielíńska, Elzbieta |
| Copyright Year | 2008 |
| Abstract | Gomulka P., T. Wlasow, J.Velíšek, Z. svobodová, e. chmielinska: effects of eugenol and ms-222 anaesthesia on siberian sturgeon Acipenser baerii brandt. acta Vet. brno 2008, 77: 447-453. The aim of the present study was to assess changes induced in the organism of siberian sturgeon (Acipenser baerii) by eugenol and ms-222 anaesthesia on the basis of haematological indices, biochemical blood profile values and histological examinations. The haematological and biochemical indices were assessed in fish immediately and 24 h after anaesthesia. The results showed that despite no mortality occurred after anaesthesia in both 125 mg·l-1 of ms-222 and 0.075 ml·l-1 of eugenol, these chemical substances severely influenced the constituents of Siberian sturgeon blood and resulted in some histological changes in the gills and liver. both eugenol and ms-222 anaesthesia caused erythrocyte swelling and haemolysis. The severe depletion of leukocyte number occurred 24 h after both eugenol and ms-222 anaesthesia (mainly due to depletion of lymphocyte, neutrophil segments and eosinophil fractions). Total protein, total globulin, triacylglycerol concentration and alanine aminotransferase activity in blood plasma was significantly elevated (p < 0.01) after both eugenol and ms-222 anaesthesia. The concentration of ca2+, inorganic phosphate, nh3 and alkaline phosphatase activity were significantly decreased (p < 0.01) compared to control. No significant changes were noticed in the albumin and glucose concentrations and the activity of lactate dehydrogenase, aspartate aminotrasferase and creatinkinase. Results of the examinations suggest that the use of MS-222 (125 mg·l-1) and eugenol (0.075 ml·l-1) does not cause irreversible damage in siberian sturgeon. Anaesthetics, blood, stress, Acipenseridae sturgeons are considered to be ‘‘living fossils’’ (bemis et al. 1997). Primitive characteristics, such as a heterocercal tail and cartilaginous skeleton, have been maintained over approximately 100 200 million years despite major environmental changes (baker et al. 2005; asadi et al. 2006). sturgeons have undergone multiple genome duplications during their evolution, which may account for their resistance to deleterious mutations, since there are probably several functional copies of every gene (blacklidge and bidwell 1993). Their primitive characteristics make sturgeons intriguing animals for study, since their biochemical haematological profile may differ substantially from the teleost profile. all sturgeon species worldwide are covered under the provisions of ciTes. several species are considered threatened with extinction as a result of over-fishing, poaching, water pollution, damming and destruction of natural watercourses and habitats (anonymous 2002). The culture of sturgeons is one of the on-growing aquaculture branch in europe due to the need of active protection of natural populations and the high demand for caviar. usually the big size and sharp bony shields on the body surface make handling of sturgeon spawners difficult and dangerous for the operating personnel. The use of general anaesthetics is a common practise in sturgeon culture, especially during artificial Address for correspondence: dr Piotr Gomulka university of Warmia and mazury department of ichthyology oczapowskiego 5 10719 olsztyn, Poland Phone: + 480 895 234 225 Fax: + 480 895 233 754 e-mail: pgomulka@uwm.edu.pl http://www.vfu.cz/acta-vet/actavet.htm ACTA VET. BRNO 2008, 77: 447–453; doi:10.2754/avb200877030447 |
| Starting Page | 447 |
| Ending Page | 453 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| DOI | 10.2754/avb200877030447 |
| Volume Number | 77 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
