NDLI: Identification of Variable-Number Tandem-Repeat (VNTR) Sequences in Acinetobacter pittii and Development of an Optimized Multiple-Locus VNTR Analysis Typing Scheme.

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Identification of Variable-Number Tandem-Repeat (VNTR) Sequences in Acinetobacter pittii and Development of an Optimized Multiple-Locus VNTR Analysis Typing Scheme.

Content Provider	Semantic Scholar
Author	Hu, Yuan Lian Jin, Da Zhi He, Li Hua Tao, Xiao Xia Zhang, Jian
Copyright Year	2015
Abstract	OBJECTIVE To develop a multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) assay for Acinetobacter pittii typing. METHODS Polymorphic VNTRs were searched by Tandem Repeats Finder. The distribution and polymorphism of each VNTR locus were analyzed in all the A. pittii genomes deposited in the NCBI genome database by BLAST and were evaluated with a collection of 20 well-characterized clinical A. pittii strains and one reference strain. The MLVA assay was compared with pulsed-field gel electrophoresis (PFGE) for discriminating A. pittii isolates. RESULTS Ten VNTR loci were identified upon bioinformatic screening of A. pittii genomes, but only five of them showed full amplifiability and good polymorphism. Therefore, an MLVA assay composed of five VNTR loci was developed. The typeability, reproducibility, stability, discriminatory power, and epidemiological concordance were excellent. Compared with PFGE, the new optimized MLVA typing scheme provided the same and even greater discrimination. CONCLUSION Compared with PFGE, MLVA typing is a faster and more standardized alternative for studying the genetic relatedness of A. pittii isolates in disease surveillance and outbreak investigation.
Starting Page	610
Ending Page	615
Page Count	6
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.besjournal.com/Articles/Archive/2015/No12/201601/P020160114536182600210.pdf
PubMed reference number	26777905v1
Alternate Webpage(s)	https://doi.org/10.3967/bes2015.119
DOI	10.3967/bes2015.119
Journal	Biomedical and environmental sciences : BES
Volume Number	28
Issue Number	12
Language	English
Access Restriction	Open
Subject Keyword	Acinetobacter calcoaceticus Bio-Informatics Common Variable Immunodeficiency Electrophoresis, Gel, Pulsed-Field Epidemiology Ethanol 0.62 ML/ML Topical Gel Gel Electrophoresis (lab technique) Genome NCBI Taxonomy Search - action Tandem Repeat Variation
Content Type	Text
Resource Type	Article

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