NDLI: Molecular characterization of the Aspergillus nidulans fbxA encoding an F-box protein involved in xylanase induction.

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Molecular characterization of the Aspergillus nidulans fbxA encoding an F-box protein involved in xylanase induction.

Content Provider	Semantic Scholar
Author	Colabardini, Ana Cristina Humanes, Ana Carolina Gouvêa, Paula Fagundes De Savoldi, Marcela Goldman, Maria Helena S. Kress, Márcia Regina Von Zeska Bayram, Özgür Oliveira, Juliana Velasco De Castro Gomes, Marcelo Damário Braus, Gerhard H. Goldman, Gustavo Henrique
Copyright Year	2012
Abstract	The filamentous fungus Aspergillus nidulans has been used as a fungal model system to study the regulation of xylanase production. These genes are activated at transcriptional level by the master regulator the transcriptional factor XlnR and repressed by carbon catabolite repression (CCR) mediated by the wide-domain repressor CreA. Here, we screened a collection of 42 A. nidulans F-box deletion mutants grown either in xylose or xylan as the single carbon source in the presence of the glucose analog 2-deoxy-D-glucose, aiming to identify mutants that have deregulated xylanase induction. We were able to recognize a null mutant in a gene (fbxA) that has decreased xylanase activity and reduced xlnA and xlnD mRNA accumulation. The ΔfbxA mutant interacts genetically with creAd-30, creB15, and creC27 mutants. FbxA is a novel protein containing a functional F-box domain that binds to Skp1 from the SCF-type ligase. Blastp analysis suggested that FbxA is a protein exclusive from fungi, without any apparent homologs in higher eukaryotes. Our work emphasizes the importance of the ubiquitination in the A. nidulans xylanase induction and CCR. The identification of FbxA provides another layer of complexity to xylanase induction and CCR phenomena in filamentous fungi.
File Format	PDF HTM / HTML
DOI	10.1016/j.fgb.2011.11.004
PubMed reference number	22142781
Journal	Medline
Volume Number	49
Issue Number	2
Alternate Webpage(s)	http://eprints.maynoothuniversity.ie/6947/1/OB-Molecular-Characterisation.pdf
Alternate Webpage(s)	https://doi.org/10.1016/j.fgb.2011.11.004
Journal	Fungal genetics and biology : FG & B
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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