Emission Polarization of Europium and Terbium Chelates

Content Provider	Semantic Scholar
Author	Reifenberger, Jeff G. Snyder, Gregory E. Baym, Gordon Selvin, Paul R.
Abstract	We measured the anisotropy of Tb 3+ and Eu3+ in various chelate complexes bound with or without a carbostyril antenna. The anisotropy of the lanthanides depends on the chelate as well as whether the antenna is present. The anisotropy of Eu 3+ bound to only a chelate is high while for Tb 3+ the anisotropy is nearly zero. For Eu3+, the anisotropy decreases upon addition of the antenna, while for Tb 3+ the anisotropy slightly increases once the antenna is attached. A theoretical model is discussed to account for the measured anisotropy of the Eu3+ bound to the chelate and how the addition of the antenna affects the anisotropy. While the transitions within Tb3+ are more complicated than those in Eu 3+, the theoretical model is also extended to Tb 3+ to explain qualitatively how the addition of the antenna affects the measured anisotropy of the terbium complex. Because of their low anisotropy, both Tb 3+ and Eu3+, when bound to a chelate with an antenna, are ideal luminescent probes in resonance energy transfer experiments for measuring nanometer scale distances. Our results indicate that it may be possible to construct a probe with Eu 3+ that retains its high anisotropy once the antenna is added to the chelate complex. This probe could measure rotational motion on the millisecond lifetime.
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Alternate Webpage(s)	http://people.physics.illinois.edu/Selvin/PRS/PSCV/PTP/2003/Jeff's%20jp0357424%20Ln%20emission%20polarization%20JPC%20B%202003.pdf
Language	English
Access Restriction	Open
Subject Keyword	Acceptor (semiconductors) Anisotropy Antenna Device Component Arabic numeral 0 Assumed Chelating Agents Distance Dyes Ehrlich units per deciliter Energy Transfer European Union Europium Experiment Gadolinium DTPA Lanthanoid Series Elements Pentetic Acid Perturbation theory (quantum mechanics) Pleural Tuberculosis Polarization (waves) Resonance Smart antenna Technetium Tc 99m Pentetate Terabyte Terbium Theory carbostyril
Content Type	Text
Resource Type	Article