Estandarización de un protocolo de recolección de muestras y PCR en tiempo real para la detección e identificación de especies de leptospira patógenas en muestras de agua de río.

Content Provider	Semantic Scholar
Author	Granja, Gutiérrez Miguel, Bernardo
Copyright Year	2013
Abstract	Leptospirosis is a zoonotic disease of worldwide distribution, and a considerable public health problem in Ecuador. This disease, caused by bacteria from the genus Leptospira, is commonly transmitted through water and soil contaminated with urine from infected mammals. For this reason, monitoring commonly usable water sources from risk areas is an important tool for understanding the development of leptospirosis outbreaks. This study presents a conventional PCR and real time PCR strategy for the detection of pathogenic species of Leptospira in water samples from two Ecuadorian provinces. For this purpose, Leptospira specific primers were used targeting the secY and lipL32 genes. Additionally, the presence of intermediate specieswas determined using primers that target a sequence of the 16S ribosomal gene. Our results show the presence of pathogenic species in 8 samples: 6 corresponding to the province of Esmeraldas and 2 corresponding to the Province of Manabi. The sequence analysis of these samples for the lipL32 PCR products allowed for the identification of the species present in 8 out of 9 samples. We also found the presence of 13 positive water samples for intermediate species, out of which 9 samples were also positive for pathogenic species of Leptospira. The results of this project provide with an important tool for future studies of pathogenic Leptospira monitoring in water sources.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://repositorio.usfq.edu.ec/bitstream/23000/2504/1/107010.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article