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Selective excitation of tryptophan fluorescence decay in proteins using a sub-nanosecond 295 nm light-emitting diode and time-correlated single-photon counting
| Content Provider | Semantic Scholar |
|---|---|
| Author | Mcguiness, Claire Sagoo, Kulwinder McLoskey, David Birch, David J. S. |
| Copyright Year | 2005 |
| Abstract | We demonstrate an AlGaN light-emitting diode (LED) giving pulses of ∼600ps full width half maximum, 0.35μW average power, 0.6mW peak power, and ∼12nm bandwidth at 295nm. This source is ideal for protein intrinsic tryptophan fluorescence decay research without the unwanted excitation of tyrosine and paves the way to lab-on-a-chip protein assays using fluorescence decay times. Fluorescence decay and anisotropy decay measurements of human serum albumin are reported and the usefulness of the 295nm LED demonstrated in comparisons with a nanosecond flashlamp and LEDs with nominal wavelength emission of 280nm. |
| Starting Page | 261911 |
| Ending Page | 261911 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| DOI | 10.1063/1.1984088 |
| Alternate Webpage(s) | http://www.horiba.com/fileadmin/uploads/Scientific/Documents/Fluorescence/APL86.pdf |
| Alternate Webpage(s) | https://doi.org/10.1063/1.1984088 |
| Volume Number | 86 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
