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Human Plasma a ,-Macroglobulin and von Willebrand Factor Possess Covalently Linked ABO ( H ) Blood Group Antigens in Subjects With Corresponding A B 0 Phenotype
| Content Provider | Semantic Scholar |
|---|---|
| Author | Matsui, Taei Fujimura, Yoshihiro Nishida, Sachiyo Titani, Koiti |
| Copyright Year | 2003 |
| Abstract | We recently identified ABO(H) blood group structures in Asn-linked sugar chains of human von Willebrand factor (vWF) purified from factor Vlll concentrates (J Biol Chem 267:8723, 1992). We surveyed plasma glycoproteins carrying ABO(H) blood group antigens by Western blotting analysis and sandwich enzyme-linked immunosorbent assay using blood group-specific monoclonal antibodies (MoAbs) and a lectin. Two major plasma proteins showing apparent molecular weight of about 180 Kd and 270 Kd by sodium dodecyl sulfate polyacrylamide gel electrophoresis reacted with blood group-specific MoAbs and Ulex europaeus lectin I in accordance with donor blood group. Direct sequence analysis of the protein bands showed their identity with the N-terminal sequences of a,-macroglobulin (a,M) and vWF, respectively. The two bands also |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.bloodjournal.org/content/bloodjournal/82/2/663.full.pdf?sso-checked=true |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |