NDLI: Impact of microRNA-134 on neural cell survival against ischemic injury in primary cultured neuronal cells and mouse brain with ischemic stroke by targeting HSPA12B

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Impact of microRNA-134 on neural cell survival against ischemic injury in primary cultured neuronal cells and mouse brain with ischemic stroke by targeting HSPA12B

Content Provider	Semantic Scholar
Author	Chi, Wenying Meng, Fanjun Li, Peilong Li, Junfa
Copyright Year	2014
Abstract	As a newly discovered member of the HSP70 family, heat shock protein A12B (HSPA12B) is involved in brain ischemic injury. According to our previous study, microRNA-134 (miR-134) could target HSPA12B by binding to its 3'-untranslated region (UTR). However, the regulation of miR-134 on HSPA12B and their role in protecting neuronal cells from ischemic injury are unclear. In this study, the miR-134 expression level was manipulated, and the HSPA12B protein levels were also determined in oxygen-glucose deprivation (OGD)-treated primary cultured neuronal cells in vitro and mouse brain after middle cerebral artery occlusion (MCAO)-induced ischemic stroke in vivo. The results showed that miR-134 expression levels increased in primary cultured neuronal cells and mouse brain from 12h to 7 day reoxygenation/reperfusion after 1h OGD or 1h MCAO treatment. miR-134 overexpression promoted neuronal cell death and apoptosis by decreasing HSPA12B protein levels. Conversely, downregulating miR-134 reduced neuronal cell death and apoptosis by enhancing HSPA12B protein levels. Also, HSPA12B siRNA could block miR-134 inhibitor-mediated neuroprotection against OGD-induced neuronal cell injury in vitro. Taken together, miR-134 might influence neuronal cell survival against ischemic injury in primary cultured neuronal cells and mouse brain with ischemic stroke by negatively modulating HSPA12B protein expression in a posttranscriptional manner.
Starting Page	22
Ending Page	33
Page Count	12
File Format	PDF HTM / HTML
DOI	10.1016/j.brainres.2014.09.072
Alternate Webpage(s)	https://api.elsevier.com/content/article/pii/S0006899314013456
Alternate Webpage(s)	https://www.sciencedirect.com/science/article/pii/S0006899314013456?dgcid=api_sd_search-api-endpoint
PubMed reference number	25304362
Alternate Webpage(s)	https://doi.org/10.1016/j.brainres.2014.09.072
Journal	Medline
Volume Number	1592
Journal	Brain Research
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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