Antibody responses to Campylobacter infections determined by an enzyme-linked immunosorbent assay: 2-year follow-up study of 210 patients.

Content Provider	Semantic Scholar
Author	Strid, Mette Aagaard Larsen, Leif Børge Begtrup, Kamilla Mølbak, Kåre Krogfelt, Karen Angeliki
Copyright Year	2001
Abstract	An enzyme-linked immunosorbent assay (ELISA) was adapted to measure immunoglobulin G (IgG), IgM, and IgA classes of human serum antibody to Campylobacter jejuni and Campylobacter coli. Heat-stable antigen, a combination of C. jejuni serotype O:1,44 and O:53 in the ratio 1:1, was used as a coating antigen in the ELISA test. A total of 631 sera from 210 patients with verified Campylobacter enteritis were examined at various intervals after infection, and a control group of 164 sera were tested to determine the cut-off for negative results. With a 90th percentile of specificity, IgG, IgM, and IgA showed a sensitivity of 71, 60, and 80%, respectively. By combining all three antibody classes, the sensitivity was 92% within 35 days after infection, whereas within 90 days after infection, a combined sensitivity of 90% was found (IgG 68%, IgM 52%, and IgA 76%). At follow-up of the patients, IgG antibodies were elevated 4.5 months after infection but exhibited a large degree of variation in antibody decay profiles. IgA and IgM antibodies were elevated during the acute phase of infection (up to 2 months from onset of infection). The antibody response did not depend on Campylobacter species or C. jejuni serotype, with the important exception of response to C. jejuni O:19, the serotype most frequently associated with Guillain-Barré syndrome. All of the patients infected with this serotype had higher levels of both IgM (P = 0.006) and IgA (P = 0.06) compared with other C. jejuni and C. coli serotypes.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://cvi.asm.org/content/8/2/314.full.pdf
PubMed reference number	11238214v1
Volume Number	8
Issue Number	2
Journal	Clinical and diagnostic laboratory immunology
Language	English
Access Restriction	Open
Subject Keyword	Antibody Formation Campylobacter coli Campylobacter infection Campylobacter jejuni Ab:ACnc:Pt:Ser:Qn:IA Campylobacter sp identified:Prid:Pt:Isolate:Nom:Organism specific culture Class Coating Excipient Eighty Enteritis Enzyme-Linked Immunosorbent Assay Guillain-Barre Syndrome Immunoglobulin A Immunoglobulin G Immunoglobulin Isotypes Patients Serotype
Content Type	Text
Resource Type	Article