Loading...
Please wait, while we are loading the content...
Similar Documents
In vitro methods for generating highly purified EBV associated tumor antigen-specific T cells by using solid phase T cell selection system for immunotherapy.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Li, Jongming Mookerjee, Bijoyesh Flomenberg, Neal |
| Copyright Year | 2007 |
| Abstract | Adoptive cell transfer immunotherapy has been utilized to treat EBV related human malignancies including post-transplant lymphoproliferative diseases, Hodgkin's lymphoma and nasopharyngeal carcinoma. However, there are limited options available for tumor antigen-specific T cell purification. Here we describe a novel solid phase T cell selection system, in which monocytes or EBV transformed B-lymphocytes are immobilized on solid support for antigen-specific T cell purification. We hypothesize and prove that antigen-specific T cells recognize their cognate antigens and bind to them faster than non-antigen specific T cells. Therefore antigen-specific T cells can be concentrated on the surface after removing the non-adherent cells by washing. The optimal selection time for both EBV-specific T cells and LMP2-specific T cells is studied. Our data demonstrate that the frequency of antigen-specific T cells can be increased by >20-fold after selection. Moreover, activated antigen-specific T cells proliferate more rapidly than non-specific T cells, further increasing the frequency and purity of antigen-specific T cells. This new T cell selection system is superior to traditional repeated stimulation methods in generating tumor antigen-specific T cells. We are able to generate large quantities of highly purified T cells of subdominant antigens LMP2 within 2 weeks after T cell activation for adoptive cell transfer immunotherapy with this simple, rapid and inexpensive T cell selection system. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://jdc.jefferson.edu/cgi/viewcontent.cgi?article=1000&context=medoncfp |
| Alternate Webpage(s) | http://jdc.jefferson.edu/cgi/viewcontent.cgi?article=1000&context=medoncfp |
| PubMed reference number | 17931649v1 |
| Volume Number | 328 |
| Issue Number | 1-2 |
| Journal | Journal of immunological methods |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adoptive Immunotherapy B-Lymphocytes Concentrate Dosage Form Endocrine System Diseases Epstein-Barr Virus Leukemia, B-Cell Lymphoma Lymphoma, Non-Hodgkin Lymphoproliferative Disorders Nasopharyngeal carcinoma Nasopharynx Neoplasms PSMB9 wt Allele Quantity T cell selection T-Cell Activation T-Lymphocyte Tumor Antigens Wash (cleansing action) |
| Content Type | Text |
| Resource Type | Article |
