Distribution of NF-B-binding sites across human chromosome 22

Content Provider	Semantic Scholar
Author	Martone, Rebecca Euskirchen, Ghia M. Bertone, Paul Hartman, Stephen Royce, Thomas E. Luscombe, Nicholas M. Rinn, John L. Nelson, F. Kenneth Miller, Perry Gerstein, Mark Weissman, Sherman Snyder, Michael
Copyright Year	2003
Abstract	We have mapped the chromosomal binding site distribution of a transcription factor in human cells. The NFB family of transcription factors plays an essential role in regulating the induction of genes involved in several physiological processes, including apoptosis, immunity, and inflammation. The binding sites of the NFB family member p65 were determined by using chromatin immunoprecipitation and a genomic microarray of human chromosome 22 DNA. Sites of binding were observed along the entire chromosome in both coding and noncoding regions, with an enrichment at the 5 end of genes. Strikingly, a significant proportion of binding was seen in intronic regions, demonstrating that transcription factor binding is not restricted to promoter regions. NFB binding was also found at genes whose expression was regulated by tumor necrosis factor , a known inducer of NFB-dependent gene expression, as well as adjacent to genes whose expression is not affected by tumor necrosis factor . Many of these latter genes are either known to be activated by NFB under other conditions or are consistent with NFB’s role in the immune and apoptotic responses. Our results suggest that binding is not restricted to promoter regions and that NFB binding occurs at a significant number of genes whose expression is not altered, thereby suggesting that binding alone is not sufficient for gene activation.
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Alternate Webpage(s)	http://archive.gersteinlab.org/papers/e-print/martone-pnas-nfkb-chipchip/reprint.pdf
Alternate Webpage(s)	http://archive.gersteinlab.org/papers/e-print/martone-pnas-nfkb-chipchip/revised.pdf
Language	English
Access Restriction	Open
Subject Keyword	Apoptosis Binding Sites Chromosomes, Human, Pair 22 DNA binding site ErbB Receptors Gene Expression Gene Ontology Term Enrichment Inducer Introns Mathematical induction Microarray NF-kappa B Name binding Neurofeedback Physiological Processes Promoter Regions, Genetic TRANSCRIPTION FACTOR Transcription (software) chromatin immunoprecipitation tumor necrosis
Content Type	Text
Resource Type	Article