Loading...
Please wait, while we are loading the content...
Similar Documents
UvA-DARE ( Digital Academic Repository ) Moving towards improved malaria control
| Content Provider | Semantic Scholar |
|---|---|
| Copyright Year | 2018 |
| Abstract | Background Artemisinin-based combinations differ in their impact on gametocyte prevalence and density. This study assessed female and male gametocyte dynamics after treating children with uncomplicated Plasmodium falciparum malaria with either pyronaridine-artesunate (PA) or artemether-lumefantrine (AL). Methods Kenyan children with uncomplicated Plasmodium falciparum malaria were included and randomly assigned to PA or AL treatment. Filter paper blood samples were collected as a source of RNA for quantitative reverse-transcription PCR (qRT-PCR) and nucleic acid sequence based amplification (QT-NASBA) to detect female gametocytes (targeting Pfs25 mRNA). Male gametocytes were detected by qRT-PCR (targeting PfMGET mRNA). Duration of gametocyte carriage, the female and male gametocyte response and the agreement between qRT-PCR and QT-NASBA were determined. Results The mean duration of female gametocyte carriage was significantly longer for PA (4.9 days) than for AL (3.8 days) as estimated by QT-NASBA (P=0.036), but this difference was less clear when determined by Pfs25 qRT-PCR (4.5 days for PA and 3.7 for AL, P=0.166). qRT-PCR based female gametocyte prevalence decreased from 100% (75/75) at baseline to 6.06% (4/66) at day 14 in the AL group and from 97.7% (83/85) to 13.9% (11/79) in the PA group. Male gametocyte prevalence decreased from 41.3% (31/75) at baseline to 19.7% (13/66) at day 14 in the AL group and from 35.3% (30/85) to 22.8% (18/79) in the PA group. There was good agreement between Pfs25 qRT-PCR and QTNASBA female gametocyte prevalence (0.85, 95% CI: 0.82 – 0.87). Conclusions This study indicates that female gametocyte clearance may be slightly faster after AL compared to PA. Male gametocytes showed similar post-treatment clearance between study arms. Future studies should further address potential differences between the post-treatment transmission potential after PA compared to AL. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://pure.uva.nl/ws/files/29079438/Chapter_7.pdf |
| Alternate Webpage(s) | https://pure.uva.nl/ws/files/29079432/Chapter_4.pdf |
| Alternate Webpage(s) | https://pure.uva.nl/ws/files/29079440/Chapter_8.pdf |
| Alternate Webpage(s) | https://pure.uva.nl/ws/files/29079444/Stellingen.pdf |
| Alternate Webpage(s) | https://pure.uva.nl/ws/files/29079426/Chapter_1.pdf |
| Alternate Webpage(s) | https://pure.uva.nl/ws/files/29079428/Chapter_2.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
