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Finasteride induces apoptosis via Bcl-2, Bcl-xL, Bax and caspase-3 proteins in LNCaP human prostate cancer cell line.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Golbano, Jesús Lóppez-Aparicio, Pilar Recio, María N. Pérez-Albarsanz, Miguel A. |
| Copyright Year | 2008 |
| Abstract | We investigated the effects of finasteride, a 5alpha-reductase inhibitor, on cell death machinery through the induction of apoptosis in an in vitro model for prostate cancer. Finasteride treatment of the LNCaP hormone-dependent human prostate cancer cell line caused the loss of cell viability and accelerated apoptosis in a concentration-dependent manner. The contents of immunoreactive procaspase-3 were examined by immunoblot analysis and the results suggest that the apoptosis induced by finasteride involves the increase of caspase-3 activity. Early cell changes that occur during apoptosis are associated with mitochondrial changes mediated by members of the Bcl-2 family of proteins. Therefore, Bcl-2, Bcl-xL and Bax were evaluated by the Western blot analysis. The immunoreactivity for pro-apoptotic Bax was markedly increased whereas antiapoptotic Bcl-2 and Bcl-xL expression was significantly reduced after incubation of cells with finasteride. These findings suggest that finasteride induces apoptosis in LNCaP cells via proteins of the Bcl-2 and caspase family. |
| File Format | PDF HTM / HTML |
| DOI | 10.3892/ijo.32.4.919 |
| PubMed reference number | 18360719 |
| Journal | Medline |
| Volume Number | 32 |
| Issue Number | 4 |
| Journal | International journal of oncology |
| Alternate Webpage(s) | https://www.spandidos-publications.com/ijo/32/4/919/download |
| Alternate Webpage(s) | https://doi.org/10.3892/ijo.32.4.919 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
