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Finding immune gene expression differences induced by marine bacterial pathogens in the Deep-sea hydrothermal vent mussel Bathymodiolus azoricus
| Content Provider | Semantic Scholar |
|---|---|
| Author | Martins, Eva Queiroz, Augusto César De Santos, Ricardo Serrão Bettencourt, Raul |
| Copyright Year | 2013 |
| Abstract | The deep-sea hydrothermal vent mussel Bathy- modiolus azoricus lives in a natural environment charac- terised by extreme conditions of hydrostatic pressure, tem- perature, pH, high concentrations of heavy metals, methane and hydrogen sulphide. The deep-sea vent biological sys- tems represent thus the opportunity to study and provide new insights into the basic physiological principles that gov- ern the defense mechanisms in vent animals and to under- stand how they cope with microbial infections. Hence, the importance of understanding this animal's innate defense mechanisms, by examining its differential immune gene ex- pressions toward different pathogenic agents. In the present study, B. azoricus mussels were infected with single sus- pensions of marine bacterial pathogens, consisting of Vib- rio splendidus, Vibrio alginolyticus,or Vibrio anguillarum, and a pool of these Vibrio bacteria. Flavobacterium suspen- sions were also used as a non-pathogenic bacterium. Gene expression analyses were carried out using gill samples from infected animals by means of quantitative-Polymerase Chain Reaction aimed at targeting several immune genes. We also performed SDS-PAGE protein analyses from the same gill tissues. We concluded that there are different levels of immune gene expression between the 12 h to 24 h exposure times to various bacterial suspensions. Our results from qPCR demonstrated a general pattern of gene expression, decreas- ing from 12 h over 24 h post-infection. Among the bacteria tested, Flavobacteriumis the bacterium inducing the highest gene expression level in 12 h post-infections animals. The 24 h infected animals revealed, however, greater gene ex- pression levels, using V. splendidus as the infectious agent. The SDS-PAGE analysis also pointed at protein profile dif- ferences between 12 h and 24 h, particularly evident for pro- teins of 18-20 KDa molecular mass, where most dissimilar- ity was found. Multivariate analyses demonstrated that im- mune genes, as well as experimental infections, clustered in discrete groups in accordance with the gene expression pat- terns induced by bacterial pathogens. |
| Starting Page | 7279 |
| Ending Page | 7291 |
| Page Count | 13 |
| File Format | PDF HTM / HTML |
| DOI | 10.5194/bg-10-7279-2013 |
| Volume Number | 10 |
| Alternate Webpage(s) | https://www.biogeosciences-discuss.net/10/C1324/2013/bgd-10-C1324-2013.pdf |
| Alternate Webpage(s) | https://www.biogeosciences-discuss.net/10/C2360/2013/bgd-10-C2360-2013.pdf |
| Alternate Webpage(s) | https://www.biogeosciences.net/10/7279/2013/bg-10-7279-2013.pdf |
| Alternate Webpage(s) | https://www.biogeosciences-discuss.net/10/C1462/2013/bgd-10-C1462-2013.pdf |
| Alternate Webpage(s) | https://www.biogeosciences-discuss.net/10/2675/2013/bgd-10-2675-2013.pdf |
| Alternate Webpage(s) | https://www.biogeosciences-discuss.net/10/C2360/2013/bgd-10-C2360-2013-print.pdf |
| Alternate Webpage(s) | http://www.biogeosciences-discuss.net/10/2675/2013/bgd-10-2675-2013.pdf |
| Alternate Webpage(s) | https://www.biogeosciences-discuss.net/10/C1324/2013/bgd-10-C1324-2013-print.pdf |
| Alternate Webpage(s) | https://www.biogeosciences-discuss.net/10/C1462/2013/bgd-10-C1462-2013-print.pdf |
| Alternate Webpage(s) | https://doi.org/10.5194/bg-10-7279-2013 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
