NDLI: Polynucleotide phosphorylase negatively controls spv virulence gene expression in Salmonella enterica.

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Polynucleotide phosphorylase negatively controls spv virulence gene expression in Salmonella enterica.

Content Provider	Semantic Scholar
Author	Ygberg, Sofia Eriksson Clements, Mark O. Rytkönen, Anne Thompson, A. Holden, David William Hinton, Jay C. D. Rhen, Mikael
Copyright Year	2006
Abstract	Mutational inactivation of the cold-shock-associated exoribonuclease polynucleotide phosphorylase (PNPase; encoded by the pnp gene) in Salmonella enterica serovar Typhimurium was previously shown to enable the bacteria to cause chronic infection and to affect the bacterial replication in BALB/c mice (M. O. Clements et al., Proc. Natl. Acad. Sci. USA 99:8784-8789, 2002). Here, we report that PNPase deficiency results in increased expression of Salmonella plasmid virulence (spv) genes under in vitro growth conditions that allow induction of spv expression. Furthermore, whole-genome microarray-based transcriptome analyses of bacteria growing inside murine macrophage-like J774.A.1 cells revealed six genes as being significantly up-regulated in the PNPase-deficient background, which included spvABC, rtcB, entC, and STM2236. Mutational inactivation of the spvR regulator diminished the increased expression of spv observed in the pnp mutant background, implying that PNPase acts upstream of or at the level of SpvR. Finally, competition experiments revealed that the growth advantage of the pnp mutant in BALB/c mice was dependent on spvR as well. Combined, our results support the idea that in S. enterica PNPase, apart from being a regulator of the cold shock response, also functions in tuning the expression of virulence genes and bacterial fitness during infection.
File Format	PDF HTM / HTML
DOI	10.1128/IAI.74.2.1243-1254.2006
PubMed reference number	16428774
Journal	Medline
Volume Number	74
Issue Number	2
Alternate Webpage(s)	http://iai.asm.org/content/74/2/1243.full.pdf
Alternate Webpage(s)	https://doi.org/10.1128/IAI.74.2.1243-1254.2006
Journal	Infection and immunity
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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