NDLI: Glycopolymer–peptide bioconjugates with antioxidant activity via RAFT polymerization

Please wait, while we are loading the content...

Glycopolymer–peptide bioconjugates with antioxidant activity via RAFT polymerization

Content Provider	Semantic Scholar
Author	Shi, Haiting Liu, Li da Wang, Xiao-Bei Li, Jingyi
Copyright Year	2012
Abstract	Well-defined protected glycopolymer poly(2-(2,3,4,6-tetra-O-acetyl-β-D-glucosyloxy)ethyl methacrylate) (PAcGlcEMA) is synthesized by reversible addition–fragmentation chain transfer (RAFT) radical polymerization. In the presence of ethanolamine and 2,2′-dithiodipyridine, PAcGlcEMA-PDS with end thiol-reactive pyridyldisulfide (PDS) group is generated by a one-pot approach. After deprotection of PAcGlcEMA, tripeptide reduced glutathione (GSH) is conjugated to PGlcEMA-PDS by thiol–disulfide exchange reaction under mild conditions. Fluorescent assay and TEM studies demonstrate that the pendant glucose residues on PGlcEMA have specific recognition with protein Concanavalin A (Con A). In comparison to PGlcEMA-PDS, the bioconjugation of GSH to PGlcEMA enhances the affinity of glycopolymer to Con A. The association constant (Ka) for the interaction of PGlcEMA-GSH bioconjugate and Con A is 6.1 × 103 M−1, displaying a high glycoside cluster effect. The disulfide-linked GSH bioconjugate PGlcEMA-GSH shows antioxidant activity. The radical scavenging activity is validated in vitro by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. It is found that the scavenging activity is dependent on reaction time and temperature. A sustained radical scavenging activity is observed at 37 °C, and about 50% DPPH radical is cleared after 24 h incubation. The recognition and the antioxidant activity make the peptide–glycopolymer bioconjugate PGlcEMA-GSH have potential for biodetection, biomimetics and targeted antioxidant delivery.
Starting Page	1182
Ending Page	1188
Page Count	7
File Format	PDF HTM / HTML
DOI	10.1039/C2PY20021J
Alternate Webpage(s)	http://www.rsc.org/suppdata/py/c2/c2py20021j/c2py20021j.pdf
Alternate Webpage(s)	https://doi.org/10.1039/C2PY20021J
Volume Number	3
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in