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Fine particle matters induce DNA damage and G2/M cell cycle arrest in human bronchial epithelial BEAS-2B cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wu, Jin-Ming Shi, Yanfeng Asweto, Collins Otieno Feng, Lin Yang, Xiaozhe Zhang, Yan-Nan Hu, Hejing Duan, Junchao Sun, Zhiwei |
| Copyright Year | 2017 |
| Abstract | There is compelling evidence that exposure to particulate matter (PM) is linked to lung tumorigenesis. However, there is not enough experimental evidence to support the specific mechanisms of PM2.5-induced DNA damage and cell cycle arrest in lung tumorigenesis. In this study, we investigated the toxic effects and molecular mechanisms of PM2.5 on bronchial epithelial (BEAS-2B) cells. PM2.5 exposure reduced cell viability and enhanced LDH activity. The cell growth curves of BEAS-2B cells decreased gradually with the increase in PM2.5 dosage. A significant increase in MDA content and a decrease in GSH-Px activity were observed. The generation of ROS was enhanced obviously, while apoptosis increased in BEAS-2B cells exposed to PM2.5 for 24 h. DNA damage was found to be more severe in the exposed groups compared with the control. For in-depth study, we have demonstrated that PM2.5 stimulated the activation of HER2/ErbB2 while significantly upregulating the expression of Ras/GADPH, p-BRAF/BRAF, p-MEK/MEK, p-ERK/ERK, and c-Myc/GADPH in a dose-dependent manner. In summary, we suggested that exposure to PM2.5 sustained the activation of HER2/ErbB2, which in turn promoted the activation of the Ras/Raf/MAPK pathway and the expression of the downstream target c-Myc. The overexpression of c-Myc may lead to G2/M arrest and aggravate the DNA damage and apoptosis in BEAS-2B after exposure to PM2.5. |
| Starting Page | 25071 |
| Ending Page | 25081 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s11356-017-0090-3 |
| Alternate Webpage(s) | http://www.repository.embuni.ac.ke/bitstream/handle/123456789/2090/Fine%20particle%20matters%20induce%20DNA%20damage%20and%20G2M%20cell%20cycle%20arrest%20in%20human%20bronchial%20epithelial%20BEAS-2B%20cells.pdf?isAllowed=y&sequence=1 |
| PubMed reference number | 28921051 |
| Alternate Webpage(s) | https://doi.org/10.1007/s11356-017-0090-3 |
| Journal | Medline |
| Volume Number | 24 |
| Journal | Environmental Science and Pollution Research |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
