Probe generated by Y chromosome microdissection in useful for analysing the sex chromosomes of the domestic horse

Content Provider	Semantic Scholar
Author	Pieńkowska-Schelling, A. Bungo, Michael W. Owczarek-Lipska, Marta Schelling, Claude Słota, Ewa
Copyright Year	2006
Abstract	Microdissection of chromosomes is an invaluable tool for physical isolation of single chromosomes, chromosome arms or chromosome bands. In the present study we have generated a fluorescence in situ hybridization (FISH) probe from the microdissected Y chromosome of the domestic horse. For the identification of the Y chromosome, we used the CBG technique. A biotinlabelled probe in the FISH technique gave a specific signal on all Y chromosomes and an additional signal in the interstitial part of the long arm of the X chromosome. This area showed an additional C band detected by the CBG method and characteristic for this chromosome. The probe obtained can therefore be used for analysis of the Y and X chromosomes and their copies in the genome, and, in the case of the X chromosome, also for gene mapping.
Starting Page	173
Ending Page	178
Page Count	6
File Format	PDF HTM / HTML
DOI	10.22358/jafs/66878/2006
Volume Number	15
Alternate Webpage(s)	http://www.jafs.com.pl/pdf-66878-6156?filename=Probe+generated+by+Y.pdf
Alternate Webpage(s)	https://doi.org/10.22358/jafs%2F66878%2F2006
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article