Development of a Real Time Rt-pcr Assay for Detecting Genetically Different Pepino Mosaic Virus Isolates

Content Provider	Semantic Scholar
Author	Hasiów, Beata Borodynko, Natasza Pospieszny, Henryk
Copyright Year	2008
Abstract	Over a period of few years, Pepino mosaic virus (PepMV) has become one of the most important viral pathogen in tomato production worldwide. So far, five PepMV genotypes (EU, LP, CH2, US2 and US1) have been detected. A real time reverse transcription polymerase chain reaction (RTPCR) procedure, using the fluorescence dye SYBR Green was developed for a rapid and reliable detection of genetically diverse Pepino mosaic virus isolates. This procedure was used for the detection and identification of PepMV in both Solanum lycopersicum and Nicotiana benthamiana species. The melting temperature (Tm) for members of a particular strain was very similar, with a host effect that did not hinder strain identification. Under optimal reaction conditions, sensitivity of the detection was as low as 100 fg of viral RNA from infected plants. This level of sensitivity indicated that the real time RT-PCR developed in the present study could be used for routine plant health assays.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.degruyter.com/downloadpdf/j/jppr.2008.48.issue-3/v10045-008-0038-1/v10045-008-0038-1.pdf
Alternate Webpage(s)	http://www.plantprotection.pl/pdf/48(3)/jppr_48(3)_04_hasiow.pdf
Language	English
Access Restriction	Open
Subject Keyword	Dyes Fluorescence Genotype HIV Indocyanine Green Lycopersicon esculentum Mosaic - computer software Mosaic Organism Mosaic Viruses Murine sarcoma viruses Nicotiana benthamiana Pathogenic organism Pepino mosaic virus RNA Reverse Transcriptase Polymerase Chain Reaction Reverse Transcription Transcription (software) Usher Syndrome, Type I Windows RT viral capsid secondary envelopment
Content Type	Text
Resource Type	Article