Loading...
Please wait, while we are loading the content...
Similar Documents
The differentiation of human multipotent adult progenitor cells into hepatocyte-like cells induced by coculture with human hepatocyte line L02
| Content Provider | Semantic Scholar |
|---|---|
| Author | Mu, Ning Liu, Hong-Bao Meng, Qiu-Hong Du, De-Wei Jiang, Yi Hu, Huan-Zhang |
| Copyright Year | 2015 |
| Abstract | PURPOSE The aim of this study was to establish an in vitro method to purify human multipotent adult progenitor cells (hMAPCs) and assess their possible differentiation into hepatocytes by coculture with human hepatocyte line L02. METHODS hMAPCs were isolated by magnetic activated cell sorting (MACS) depletion selection using CD45 and GlyA microbeads. After indirect or direct coculture of hMAPCs and human hepatocyte line L02, the expression of albumin (ALB), alpha-fetoprotein (AFP), cytokeratin (CK) 18, and CK19 by hMAPCs was detected by immunocytochemistry. RESULTS With the MACS method, (5-10) × 10(4)/mL hMAPCs could be separated from 1 × 10(6)/mL bone marrow mononuclear cells. The purity of CD45-/GlyA- cells separated from bone marrow adherent cells was more than 98%, as determined by flow cytometry. In the coculture without cell-to-cell contact, hMAPCs expressed high AFP on day 1, and then tapered daily to low expression on day 7; ALB expression reached its peak on day 5, and remained high on day 7; CK18 was initially expressed on day 5 and was higher on day 7; CK19 was negative in all assays. In the coculture with cell-to-cell contact, ALB and CK18 were expressed by most cells while AFP appeared in only a few on day 5. CONCLUSION hMAPCs were induced to differentiate into mature hepatocyte-like cells by coculture with a hepatocyte cell line, either with or without cell-to-cell contact, but the former seemed more effective. |
| Starting Page | 1 |
| Ending Page | 7 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| DOI | 10.4174/astr.2015.88.1.1 |
| PubMed reference number | 25553318 |
| Journal | Medline |
| Volume Number | 88 |
| Alternate Webpage(s) | https://thesurgery.or.kr/Synapse/Data/PDFData/6037ASTR/astr-88-1.pdf |
| Alternate Webpage(s) | https://doi.org/10.4174/astr.2015.88.1.1 |
| Journal | Annals of surgical treatment and research |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
