Additional crystal forms of the E. coli class II fructose-1,6-bisphosphate aldolase.

Content Provider	Semantic Scholar
Author	Kitagawa, Yuji Leonard, Gordon A. Harrop, Stephen J. Peterson, Mark R. Hunter, William N. Qamar, Seema S. Berry A.
Copyright Year	1995
Abstract	We have obtained two additional crystal forms of the metal-dependent class II fructose-1,6-bisphosphate aldolase from Escherichia coli. Crystals in the shape of elongated plates have unit-cell dimensions a = 73.4, b = 120.0, c = 190.1 A, orthorhombic space group P2(1)2(1)2(1). Monoclinic prisms have unit-cell dimensions a = 67.7, b = 104.3, c = 52.8 A, beta = 105 degrees, space group P2(1). Diffraction to slightly better than 3.0 A, has been observed for both forms using in-house and synchrotron facilities. These crystal forms may aid the structure solution of this enzyme by presenting additional forms for heavy-atom derivatization. These forms have multiple copies of the enzyme in the asymmetric unit and averaging methods might also be useful in the analysis.
File Format	PDF HTM / HTML
DOI	10.1107/S0907444994013776
PubMed reference number	15299818
Journal	Medline
Volume Number	51
Part	5
Alternate Webpage(s)	http://journals.iucr.org/d/issues/1995/05/00/li0198/li0198.pdf
Alternate Webpage(s)	https://doi.org/10.1107/S0907444994013776
Journal	Acta crystallographica. Section D, Biological crystallography
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article