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Subcellular fractionation on Percoll gradient of mossy fiber synaptosomes: evoked release of glutamate, GABA, aspartate and glutamate decar☐ylase activity in control and degranulated rat hippocampus
| Content Provider | Semantic Scholar |
|---|---|
| Author | Taupin, Philippe Ben-Ari, Yezekiel Roisin, Marie-Paule |
| Copyright Year | 1994 |
| Abstract | Using discontinuous density gradient centrifugation in isotonic Percoll sucrose, we have characterized two subcellular fractions (PII and PIII) enriched in mossy fiber synaptosomes and two others (SII and SIII) enriched in small synaptosomes. These synaptosomal fractions were compared with those obtained from adult hippocampus irradiated at neonatal stage to destroy granule cells and their mossy fibers. Synaptosomes were viable as judged by their ability to release aspartate, glutamate and GABA upon K+ depolarization. After irradiation, compared to the control values, the release of glutamate and GABA was decreased by 57 and 74% in the PIII fraction, but not in the other fractions and the content of glutamate, aspartate and GABA was also decreased in PIII fraction by 62, 44 and 52% respectively. These results suggest that mossy fiber (MF) synaptosomes contain and release glutamate and GABA. Measurement of the GABA synthesizing enzyme, glutamate decarboxylase, exhibited no significant difference after irradiation, suggesting that GABA is not synthesized by this enzyme in mossy fibers. |
| Starting Page | 313 |
| Ending Page | 321 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0006-8993(94)91695-0 |
| PubMed reference number | 7914149 |
| Journal | Medline |
| Volume Number | 644 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/0006899394916950 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/0006899394916950?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/0006-8993%2894%2991695-0 |
| Journal | Brain Research |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
