NDLI: MicroRNA expression profile in intrauterine hypoxia-induced pulmonary hypoplasia in rats

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MicroRNA expression profile in intrauterine hypoxia-induced pulmonary hypoplasia in rats

Content Provider	Semantic Scholar
Author	Huo, Huiyi Luo, Ziqiang Wang, Mingjie Yu, Xiaohe Liao, Zhengchang Zhou, Xiaocheng Yue, Shaojie
Copyright Year	2014
Abstract	Hypoxia is necessary for fetal development; however, excess hypoxia is detrimental. The mechanisms underlying the effects of hypoxia on lung development remain unclear, although important roles of microRNAs (miRNAs) during lung development have recently been established. However, the effect on lung development at an miRNA expression level, following changes in oxygen tension, have not yet been studied. In the present study, pregnant rats were exposed to a fraction of inspired oxygen of 10.5 or 21% for two days on gestation day 19, following which the body weight, lung wet weight, radial alveolar count (RAC) and mean linear intercept (Lm) of the newborn pups were analyzed on postnatal day 1. To define the role of miRNAs during lung development following intrauterine hypoxia exposure, the miRNA expression pattern was profiled using a miRNA microarray. The newborn rats in the hypoxic group exhibited statistically significant decreases in body weight, lung weight and the RAC, as well as a marked increase in the Lm. A total of 69 miRNAs were identified to have significant changes in expression, including 55 upregulated and 14 downregulated miRNAs. Quantitative polymerase chain reaction was used to validate the microarray results of six selected miRNAs. Therefore, the results indicated that late gestation intrauterine hypoxia exposure may cause lung injury and miRNAs may play important roles in this process.
Starting Page	747
Ending Page	753
Page Count	7
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://ftp.ncbi.nlm.nih.gov/pub/pmc/50/2e/etm-08-03-0747.PMC4113549.pdf
Alternate Webpage(s)	https://www.spandidos-publications.com/etm/8/3/747/download
PubMed reference number	25120593v1
Volume Number	8
Journal	Experimental and therapeutic medicine
Language	English
Access Restriction	Open
Subject Keyword	Fetal development of the mammalian embryo or fetus Hypoxia Infant, Newborn Inspiration function Intrauterine Lung Injury MicroRNAs Oxygen measurement, partial pressure, arterial Polymerase Chain Reaction Pulmonary hypoplasia Structure of parenchyma of lung lung development
Content Type	Text
Resource Type	Article

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