Pertussis toxin-induced lung edema. Role of manganese superoxide dismutase and protein kinase C.

Content Provider	Semantic Scholar
Author	Tsan, Min-Fu Cao, Xiu-Jing White, James Eden Sacco, J. Lee, Candice Y.
Copyright Year	1999
Abstract	The mechanism by which pertussis toxin (Ptx) causes lung edema is not clear. We investigated the role of pulmonary manganese superoxide dismutase (MnSOD) and protein kinase C (PKC) in Ptx-induced lung edema. We demonstrated that intraperitoneal injection of Ptx at a concentration of 5 microg/100 g body weight caused a similar degree of lung edema in 2 d, as measured by lung wet weight/dry weight ratio, in heterozygous MnSOD gene (Sod2)-knockout mice (Sod2(+/-)) and in their wild-type littermates (Sod2(+/+)). The level of lung MnSOD activity in Sod2(+/-) mice was approximately half that of Sod2(+/-) mice. Ptx had no effect on levels of lung MnSOD messenger RNA, immunoreactive protein, or enzyme activity in either Sod2(+/+) or Sod2(+/-) mice. Ptx also had no effect on lung copper-zinc SOD, catalase, and glutathione peroxidase activities in these mice. On the other hand, Ptx caused the activation of lung PKC, for example, by translocation of a 72-kD PKC isoform from the cytosolic fraction to the membrane fraction. Pretreatment of mice with bisindolylmaleimide, a PKC inhibitor, prevented both the Ptx-induced activation of PKC and lung edema. These data suggest that Ptx-induced lung edema in mice is, at least in part, due to the activation of lung PKC.
File Format	PDF HTM / HTML
DOI	10.1165/ajrcmb.20.3.3373
PubMed reference number	10030845
Journal	Medline
Volume Number	20
Issue Number	3
Alternate Webpage(s)	http://intl-ajrcmb.atsjournals.org/content/20/3/465.full.pdf
Alternate Webpage(s)	https://doi.org/10.1165/ajrcmb.20.3.3373
Journal	American journal of respiratory cell and molecular biology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article