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Neuroprotective effects of salidroside against beta-amyloid-induced oxidative stress in SH-SY5Y human neuroblastoma cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhang, Li Yu, Huixin Zhao, Xincan Lin, Xiufeng Wang, Zhengwu |
| Copyright Year | 2010 |
| Abstract | Beta-amyloid (Abeta) peptide, the hallmark of Alzheimer's disease (AD), invokes a cascade of oxidative damages to neurons and eventually leads to neuronal death. In this study, salidroside (Sald), an active compound isolated from a traditional Chinese medicinal plant, Rhodiola rosea L., was investigated to assess its protective effects and the underlying mechanisms against Abeta-induced oxidative stress in SH-SY5Y human neuroblastoma cells. Abeta(25-35)-induced neuronal toxicity was characterized by the decrease of cell viability, the release of lactate dehydrogenase (LDH), morphological alterations, neuronal DNA condensation, and the cleavage of poly(ADP-ribose) polymerase (PARP) by activated caspase-3. Pretreatment with salidroside markedly attenuated Abeta(25-35)-induced loss of cell viability and apoptosis in a dose-dependent manner. The mechanisms of salidroside protected neurons from oxidative stress included the induction of antioxidant enzymes, thioredoxin (Trx), heme oxygenase-1 (HO-1), and peroxiredoxin-I (PrxI); the downregulation of pro-apoptotic protein Bax and the upregulation of anti-apoptotic protein Bcl-X(L). Furthermore, salidroside dose-dependently restored Abeta(25-35)-induced loss of mitochondrial membrane potential (MMP) as well as suppressed the elevation of intracellular reactive oxygen species (ROS) level. It was also observed that Abeta(25-35) stimulated the phosphorylation of mitogen-activated protein (MAP) kinases, including c-Jun NH(2)-terminal kinase (JNK) and p38 MAP kinase, but not extracellular signal-regulated kinase1/2 (ERK1/2). Salidroside inhibited Abeta(25-35)-induced phosphorylation of JNK and p38 MAP kinase, but not ERK1/2. These results suggest that salidroside has protective effects against Abeta(25-35)-induced oxidative stress, which might be a potential therapeutic agent for treating or preventing neurodegenerative diseases. |
| Starting Page | 547 |
| Ending Page | 555 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.neuint.2010.06.021 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/S0197018610002160 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/S0197018610002160?dgcid=api_sd_search-api-endpoint |
| PubMed reference number | 20615444 |
| Alternate Webpage(s) | https://doi.org/10.1016/j.neuint.2010.06.021 |
| Journal | Medline |
| Volume Number | 57 |
| Journal | Neurochemistry International |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
