NDLI: Identification of Glomerella cingulata f. sp phaseoli recombinants by RAPD markers.

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Identification of Glomerella cingulata f. sp phaseoli recombinants by RAPD markers.

Content Provider	Semantic Scholar
Author	Camargo, Osnil A. Souza, Elaine A. Mendes-Costa, Maria Cristina Santos, J. B. Soares, Marcelo Alves
Copyright Year	2007
Abstract	We examined the capacity of strains of Glomerella cingulata f. sp phaseoli fungus (Colletotrichum lindemuthianum sexual stage) to form recombinants, using random amplified polymorphic DNA (RAPD). Crosses of all possible combinations between strains 40, 42, 20, 21, 22, 23, 24, 25, and 26 were made on Petri dishes using M3 culture medium. The 42 x 21 cross produced the largest number of perithecia and five asci; the respective ascospores were isolated. RAPD analysis was performed on the parents and descendants. The 62 polymorphic RAPD bands obtained were used to assess the genetic similarity using the method of Sorence and Dice and clustering analysis in the form of a dendrogram by the UPGMA method. The RAPD markers allowed identification of recombinants from the cross between strains 42 and 21 of G. cingulata f. sp phaseoli and 40 ascospores presented 63 and 49% genetic similarity with parents 2 (strain 42) and 1 (strain 21), respectively.
File Format	PDF HTM / HTML
PubMed reference number	18050079
Journal	Medline
Volume Number	6
Issue Number	3
Journal	Genetics and molecular research : GMR
Alternate Webpage(s)	http://www.funpecrp.com.br/gmr/year2007/vol3-6/pdf/GMR0326.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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