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Indirect examination of exocrine pancreatic function in vivo by determination of chymotrypsin activity in animal intestine using a synthetic substrate.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kasteĺ, Rudolf Rosíval, I. Bartik, M. A. Bláhovec, Josef |
| Copyright Year | 1996 |
| Abstract | Using the substrate N-acetyl-L-tyrosyl-p-aminobenzoic acid, we determined chymotrypsin activity in the small intestine of calf, pig, and poultry. Orally administered N-acetyl-L-tyrosyl-p-aminobenzoic acid is enzymatically cleaved in vivo, and the released p-aminobenzoic acid is determined by HPLC. We found that the p-aminobenzoic acid concentration in plasma and urine was significantly influenced by the feeding of soya flour. After soybean flour feeding, the p-aminobenzoic acid concentration significantly increased in the plasma of calves and hens, in contrast to pigs, where the p-aminobenzoic acid concentration significantly decreased. This shows that the oral administration of N-acetyl-L-tyrosyl-p-aminobenzoic acid with subsequent determination of p-aminobenzoic acid is suitable for the estimation of exocrine pancreatic function and for determination of changes in intestinal proteolytic activity caused by antinutritive substances. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://edoc.hu-berlin.de/oa/degruyter/cclm.1996.34.4.311.pdf |
| PubMed reference number | 8704047v1 |
| Volume Number | 34 |
| Issue Number | 4 |
| Journal | European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 4-Aminobenzoic Acid Administration, Oral Cleaved cell Intestines Small Intestinal Wall Tissue Soybeans Structure of beta Cell of islet Thioctic Acid chymotrypsin activity substance |
| Content Type | Text |
| Resource Type | Article |
