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Transcriptional and posttranscriptional regulation and endocytosis were involved in zinc oxide nanoparticle-induced interleukin-8 overexpression in human bronchial epithelial cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yan, Zhen Wu, Yong-Jun Wang, Wei Wu, Weidong |
| Copyright Year | 2014 |
| Abstract | Inhaled zinc oxide nanoparticles (ZnO-NPs) can induce lung inflammation through released inflammatory mediators, such as interleukin 8 (IL-8), from airways. However, the mechanisms underlying ZnO-NP-induced IL-8 gene expression have not been fully characterized. The transcription inhibitor actinomycin D (Act D) and the BEAS-2B cells stably overexpressing wild-type or mutated IL-8 promoter at the NFκB or C/EBPβ binding site were used to determine the involvement of transcriptional mechanisms. The effect of ZnO-NPs on IL-8 mRNA stability was examined using mRNA decay assay. The phagocytosis inhibitor cytochalasin B (CB) was utilized to define the role of endocytosis in ZnO-NP-induced IL-8 expression. In addition, the solubility of ZnO-NPs in culture medium was assessed using atomic absorption spectroscopy. Exposure to ZnO-NPs significantly increased the expression of IL-8 mRNA and protein in a dose-dependent manner. Pretreatment with Act D blocked ZnO-NP-induced IL-8 expression. Both NFκB and C/EBPβ transcription factors were required for ZnO-NP-induced IL-8 transcription. mRNA decay assay showed that ZnO-NP stimulation delayed IL-8 mRNA degradation in BEAS-2B cells. Pretreatment of BEAS-2B cells with CB blocked ZnO-NP-induced IL-8 expression by 30 %. Exposure to ZnO-NPs induced IL-8 gene expression through transcriptional activation and mRNA stabilization. Internalization of nanoparticles was partially involved in ZnO-NP-induced IL-8 expression. |
| Starting Page | 79 |
| Ending Page | 88 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s10565-014-9270-9 |
| PubMed reference number | 24554449 |
| Journal | Medline |
| Volume Number | 30 |
| Alternate Webpage(s) | http://view.pubtsg.com:8080/enpdf/1975/1975-4-25/Transcriptional%20and%20posttranscriptional%5BPMIDS24554449%5D.pdf |
| Alternate Webpage(s) | https://doi.org/10.1007/s10565-014-9270-9 |
| Journal | Cell Biology and Toxicology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
