Role of the actin cytoskeleton during respiratory burst in chemoattractant-stimulated neutrophils.

Content Provider	Semantic Scholar
Author	Bengtsson, Torbjörn Orselius, Kristina Wetterö, Jonas
Copyright Year	2006
Abstract	The aim of this study was to clarify the role of the actin cytoskeleton during chemotactic peptide fMet-Leu-Phe (fMLF)-stimulated respiratory burst in human neutrophil granulocytes. Reactive oxygen species (ROS) was measured as luminol-amplified chemiluminescence (CL) and F-actin content as bodipy phallacidin fluorescence in neutrophils treated with latrunculin B or jasplakinolide, an inhibitor and activator of actin polymerization, respectively. Latrunculin B markedly decreased, whereas jasplakinolide increased, the F-actin content in neutrophils, unstimulated or stimulated with fMLF. Latrunculin B enhanced the fMLF-triggered ROS-production more than tenfold. Jasplakinolide initially inhibited the fMLF-induced CL-response, however, caused a potent second sustained phase (>400% of control). Both actin drugs triggered a substantial CL-response when added 5-25 min after fMLF. This was also valid for chemotactic doses of fMLF, where latrunculin B and jasplakinolide amplified the ROS-production 5-10 times. By using specific signal transduction inhibitors, we found that the NADPH oxidase activation triggered by destabilization of the actin cytoskeleton occurs downstream of phospholipase C and protein kinase C but is mediated by Rho GTPases and tyrosine phosphorylation. In conclusion, rearrangements of the actin cytoskeleton are a prerequisite in connecting ligand/receptor activation, generation of second messengers and assembly of the NADPH oxidase in neutrophil granulocytes.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://liu.diva-portal.org/smash/get/diva2:255996/FULLTEXT01.pdf
Alternate Webpage(s)	http://liu.diva-portal.org/smash/get/diva2:255996/FULLTEXT01
PubMed reference number	16448823v1
Volume Number	30
Issue Number	2
Journal	Cell biology international
Language	English
Access Restriction	Open
Subject Keyword	Amine Oxidase (Copper-Containing) Chemiluminescence Chemotactic Factors Cytoskeleton DNA Sequence Rearrangement Fluorescence Guanosine Triphosphate Phosphohydrolases Leucine Ligands Luminol Microfilaments Phenylalanine Protein Kinase C Protein Kinases Reactive Oxygen Species Receptor Activation Process Respiratory Burst Signal Transduction Tyrosine Phosphorylation actin filament polymerization fMet-Leu-Phe receptor granulocyte jasplakinolide negative regulation of phospholipase C-activating G-protein coupled receptor signaling pathway neutrophil response to latrunculin B second messenger
Content Type	Text
Resource Type	Article