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Vascular endothelial growth factor B (VEGF-B) binds to VEGF receptor-1 and regulates plasminogen activator activity in endothelial cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Olofsson, Birgitta Knutsdotter Korpelainen, E. I. Pepper, Michael Sean Mandriota, Stefano J. Aase, Karin Kumar, V. Gunji, Yuji Jeltsch, Michael Shibuya, Mihoko Alitalo, Kari Eriksson, Urs |
| Copyright Year | 1998 |
| Abstract | The vascular endothelial growth factor (VEGF) family has recently expanded by the identification and cloning of three additional members, namely VEGF-B, VEGF-C, and VEGF-D. In this study we demonstrate that VEGF-B binds selectively to VEGF receptor-1/Flt-1. This binding can be blocked by excess VEGF, indicating that the interaction sites on the receptor are at least partially overlapping. Mutating the putative VEGF receptor-1/Flt-1 binding determinants Asp63, Asp64, and Glu67 to alanine residues in VEGF-B reduced the affinity to VEGF receptor-1 but did not abolish binding. Mutational analysis of conserved cysteines contributing to VEGF-B dimer formation suggest a structural conservation with VEGF and platelet-derived growth factor. Proteolytic processing of the 60-kDa VEGF-B186 dimer results in a 34-kDa dimer containing the receptor-binding epitopes. The binding of VEGF-B to its receptor on endothelial cells leads to increased expression and activity of urokinase type plasminogen activator and plasminogen activator inhibitor 1, suggesting a role for VEGF-B in the regulation of extracellular matrix degradation, cell adhesion, and migration. |
| File Format | PDF HTM / HTML |
| DOI | 10.1073/pnas.95.20.11709 |
| PubMed reference number | 9751730 |
| Journal | Medline |
| Volume Number | 95 |
| Issue Number | 20 |
| Alternate Webpage(s) | https://jeltsch.org/sites/jeltsch.org/files/OlofssonBirgitta_PNAS1998_11709.pdf |
| Alternate Webpage(s) | https://doi.org/10.1073/pnas.95.20.11709 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
