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Aldosterone regulates the Na-K-2Cl cotransporter in vascular smooth muscle.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jiang, Gengru Cobbs, Scott L. Klein, Janet D. O'neill, W. Charles |
| Copyright Year | 2003 |
| Abstract | Aldosterone increases cation transport and contractility of vascular smooth muscle, but the specific transporter involved and how it is linked to smooth muscle tone is unknown. Because the Na-K-2Cl cotransporter (NKCC1) contributes to vascular smooth muscle contraction and is regulated by vasoactive compounds, we sought to determine whether this transporter is a target of aldosterone in rat aorta. Treatment of adrenalectomized rats with aldosterone for 7 days resulted in a 63% increase in NKCC1 activity as measured by bumetanide-sensitive efflux of 86Rb+. Treatment of normal aortas in culture with aldosterone for 3 and 7 days resulted in 29% and 47% increases in NKCC1 activity, respectively. Aldosterone had no acute effect on 86Rb+ efflux. Stimulation of NKCC1 was blocked by spironolactone, a mineralocorticoid receptor antagonist, but not by RU38486, a glucocorticoid receptor antagonist. Aldosterone did not augment the stimulation of NKCC1 by phenylephrine and did not increase NKCC1 mRNA as determined by real-time polymerase chain reaction. We conclude that aldosterone regulates the Na-K-2Cl cotransporter in vascular smooth muscle through classic mineralocorticoid receptors but not through changes in the abundance of NKCC1 mRNA. This could account for the increase in Na+, K+, and Cl- fluxes previously observed in vascular smooth muscle from mineralocorticoid-treated animals and may contribute to increased vascular tone. |
| Starting Page | 557 |
| Ending Page | 560 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://hyper.ahajournals.org/content/hypertensionaha/41/5/1131.full.pdf?download=true |
| Alternate Webpage(s) | http://hyper.ahajournals.org/content/hypertensionaha/early/2003/03/31/01.HYP.0000066128.04083.CA.full.pdf?download=true |
| Alternate Webpage(s) | http://hyper.ahajournals.org/content/hypertensionaha/early/2003/03/31/01.HYP.0000066128.04083.CA.full.pdf |
| PubMed reference number | 12668585v1 |
| Volume Number | 41 |
| Issue Number | 5 |
| Journal | Hypertension |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Aldosterone Aorta Bumetanide Cations Chloride Ion Mineralocorticoid Receptor Mineralocorticoids Muscle Tonus Muscle, Smooth, Vascular Phenylephrine Polymerase Chain Reaction Potassium R-38486 SLC12A2 gene SLC12A2 wt Allele Smooth muscle (tissue) Sodium Sodium, Dietary Spironolactone Vascular Smooth Muscle Contraction Pathway |
| Content Type | Text |
| Resource Type | Article |
