NDLI: Diosgenin induces apoptosis in HepG2 cell through an ASK1-ROS-p38 MAPK/JNK cascade

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Diosgenin induces apoptosis in HepG2 cell through an ASK1-ROS-p38 MAPK/JNK cascade

Content Provider	Semantic Scholar
Author	Jeon, Byoung Kook Mun, Yeun Ja Woo, Won Hong Lee, Young-Eun
Copyright Year	2011
Abstract	Food saponins have been used in complimentary and traditional medicine against a variety of diseases including several cancers. Diosgenin, a naturally occurring steroid saponin found abundantly in legumes and yams, has been shown to have antitumor effects on cancer cells. Apoptosis signal-regulating kinase (ASK)-1, as a mitogen-activated protein kinase kinase kinase (MAPKKK), has been implicated in cytokineand stress-induced apoptosis. To elucidate the cytotoxicity mechanism of diosgenin, we investigated the role of ASK-1-p38 MAPK/JNK cascade in apoptosis and caspase-3 activation in diosgenin-treated HepG2 cells. HepG2 cells were treated with different doses of diosgenin (0 40 mM, n 3–5 per group) for 24 h at 37 C. Phosphorylated MAPK, ASK1 and caspase levels were detected by Western blotting and reactive oxygen species (ROS) production was detected by fluorescence microscopy. Results were obtained from three independent experiments.
File Format	PDF HTM / HTML
DOI	10.1017/S0029665111002655
Alternate Webpage(s)	https://www.cambridge.org/core/services/aop-cambridge-core/content/view/F0A365A88A7DC47D333BD581251AB740/S0029665111002655a.pdf/diosgenin_induces_apoptosis_in_hepg2_cell_through_an_ask1rosp38_mapkjnk_cascade.pdf
Alternate Webpage(s)	https://doi.org/10.1017/S0029665111002655
Volume Number	70
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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