NDLI: Cationic graft copolymers as carriers for delivery of antisense-oligonucleotides

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Cationic graft copolymers as carriers for delivery of antisense-oligonucleotides

Content Provider	Semantic Scholar
Author	Dautzenberg, Herbert Konák, Cestmír Reschel, Tomas Zintchenko, Arkadi Ulbrich, Karel
Copyright Year	2003
Abstract	Self-assembling system based on ionic complexes of DNA fragments (36 base pairs), bcl-2 antisense oligonucleotides (octadecamer), oligophosphates (25 phosphate groups) or acrylic oligomers (18 groups of phosphonic acid) with poly(L-lysine) (PLL) (M w = 130 000 and 88 000) grafted with short poly[N-(2-hydroxypropyl)methacrylamide] (PHPMA) chains (M n = 4 300 or 8 600) were studied by static and dynamic light scaltering methods as systems suitable for gene therapy applications. The graft copolymers (GPLLs) with shorter PHPMA grafts (M n = 4 300) provide polyelectrolyte complexes (PECs) with smaller M w and R H than the corresponding GPLLs with longer grafts (M n = 8 600) and the same content of PLL. The lowest aggregation number of 2 was observed for PECs prepared from the GPLL with short grafts and 40 wt.-% PLL. The complexes of oligonucleotides and DNA fragments with GPLLs showed quite similar behavior to that with oligophosphates and acrylic oligomer. The complexes prepared from GPLLs containing 40 wt.-% of PLL and at excess of oligophosphate were stable for at least 48h under physiological conditions (0.15 M NaCl) and in bovine serum albumin solutions (1 mg-mL -1 ). Additionally, polyanion exchange reactions of the PECs in contact with poly(styrenesulfonate) and DNA were studied in 0.15 M NaCl solutions. The oligophosphates in complexes were at least partially substituted with high molecular-weight polyanions. The structure of the initial PECs dominated the PEC structure after the exchange reaction.
Starting Page	425
Ending Page	435
Page Count	11
File Format	PDF HTM / HTML
DOI	10.1002/mabi.200350014
Volume Number	3
Alternate Webpage(s)	http://pubman.mpdl.mpg.de/pubman/item/escidoc:1929341/component/escidoc:2403477/1929341.pdf
Alternate Webpage(s)	https://doi.org/10.1002/mabi.200350014
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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