Mikrofluidischer Zellkultur-Chip mit integrierter Verdünnungsreihe für in vitro-Tests

Content Provider	Semantic Scholar
Author	Kreher, H.
Copyright Year	2016
Abstract	In this work, a microfluidic cell culture chip is developed for optimization of in vitro tests. A binary logarithmic dilution profile of an arbitrary fluid is passively generated on such a chip by a system of microchannels, reducing contaminations and enabling a broad range of applications by analyzing the interaction of different chemical compounds on cells–for example, in drug development and toxicity tests. The manufacturing process was preceded by analytical and numerical calculations, a selection of manufacturing methods as well as the development of a new convex corner compensation for anisotropic etching. Formation of air bubbles during the filling process of the chip was analyzed with respect to two different SiO 2 surface coatings. A new filling method was developed successfully, using the low pressure environment in a vacuum desiccator. Furthermore, a test setup for extracting one sample per well was engineered, and suitable methods of analysis for measuring the concentrations were investigated. The results of two chip designs are compared with each other, and the functionality of the silicon chip is demonstrated by achieving three core functions: a uniform flow rate at the outlets, the generation of a binary logarithmic dilution gradient, and the cultivation of living cells inside on-chip cavities with continuous media perfusion.
File Format	PDF HTM / HTML
DOI	10.22028/D291-23147
Alternate Webpage(s)	https://publikationen.sulb.uni-saarland.de/bitstream/20.500.11880/23203/1/Kreher_Heike_Diss_Bib.pdf
Alternate Webpage(s)	https://doi.org/10.22028/D291-23147
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article