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Tobacco mosaic virus RNA enters chloroplasts in vivo.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Schoelz, James E. Zaitlin, Milton |
| Copyright Year | 1989 |
| Abstract | Several lines of evidence are presented to allow us to conclude that tobacco mosaic virus (TMV) RNA enters the chloroplast in vivo. Chloroplasts were prepared from either directly inoculated or systemically infected leaves of tobacco plants inoculated with one of several strains of the virus and from uninfected control plants. Intact chloroplasts were isolated on Percoll gradients and treated with pancreatic RNase and thermolysin to destroy potential TMV virions and RNA on the outside or bound to their surfaces. Northern blot analysis of RNA extracted from these chloroplasts demonstrated that full-length TMV RNA was present within the chloroplasts prepared from both directly inoculated and systemically invaded leaves. Only genomic length, but not subgenomic length, RNA was found in the chloroplast extracts, indicating a selectivity of the transport of the viral RNA into the chloroplast. A temperature-sensitive TMV mutant (Ts 38), in which no virions are formed at 35 degrees C, was used to demonstrate that at that restrictive temperature viral RNA is detected in the chloroplast, indicating that free viral RNA can enter the chloroplast rather than intact virions. To our knowledge, the transport of a foreign RNA species into chloroplasts has not been reported previously. |
| Starting Page | 62 |
| Ending Page | 67 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.pnas.org/content/86/12/4496.full.pdf |
| PubMed reference number | 16578844v1 |
| Volume Number | 86 |
| Issue Number | 12 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Chloroplasts Extraction Mosaic Organism Mosaic Viruses Murine sarcoma viruses Percoll RNA RNA, Viral Thermolysin Tobacco Mosaic Virus Virion viral capsid secondary envelopment |
| Content Type | Text |
| Resource Type | Article |
