NDLI: The role of mitochondrial dysfunction in the pathophysiology of icu-acquired weakness

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The role of mitochondrial dysfunction in the pathophysiology of icu-acquired weakness

Content Provider	Semantic Scholar
Author	Jiroutková, Kateřina Žiak, Jakub Krajčová, Adéla Frič, Michal Džupa, Valér Duška, František
Copyright Year	2014
Abstract	Methods Muscle biopsies were obtained from critically ill patients with severe ICUAW (defined as MRC score < 24 on two separate tests) and from otherwise healthy controls undergoing elective hip replacement. 50-100mg of the native sample was homogenized by a technique which perserves mitochondria in their cytosolic context [3]. Oxygen consumption was measured by high-resolution respirometry (Oxygraph-2Km Oroboros) under two occasions: 1. homogenate enriched with substrates was sequentially treated with an ATPase inhibitor (oligomycine), an uncoupler (FCCP) and KCN. This allowed us to measure oxidative phosphorylation, respiratory chain capacity, proton leak through inner mitochondrial membrane and non-mitochondrial O2 consumption. 2. skeletal muscle homogenate enriched with abundant ADP was treated with sequential addition of substrates and inhibitors of complexes I, II, III, and IV, respectively. Integrity of outer membrane was assessed by measuring the response to addition of cytochrome c. Oxygen consumption rate was normalized to citrate-synthase activity and total protein content.
Starting Page	745
Ending Page	751
Page Count	7
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://icm-experimental.springeropen.com/track/pdf/10.1186/2197-425X-2-S1-P29
PubMed reference number	4797754
Volume Number	2
Language	English
Access Restriction	Open
Subject Keyword	Adenosine Diphosphate Biopsy Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone Critical Illness Hip Replacement Arthroplasty (procedure) Homogenate Image resolution Mitochondrial Diseases Mitochondrial Inheritance Mitochondrial Membranes Mitochondrial electron transport chain Muscle Weakness Oxidative Phosphorylation Oxygen Consumption Patients Protons USB On-The-Go outer membrane sodium citrate
Content Type	Text
Resource Type	Article

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