NDLI: Efeito Do Bap E Tdz Na Calogênese E Organogênese Em Internódios De Macieira Cv. Gala Rw1

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Efeito Do Bap E Tdz Na Calogênese E Organogênese Em Internódios De Macieira Cv. Gala Rw1

Content Provider	Semantic Scholar
Author	Morales, Cinara Fernanda Garcia Lombardi, Sergio Ricardo Bolte Soares, Patricia Fortes, Gerson Renan De Luces
Copyright Year	1999
Abstract	Estudou-se o efeito da benzilaminopurina (BAP) e thidiazuron (TDZ) na calogenese e organogenese de internodios de macieira, cv. Gala RW1, provenientes de cultivo in vitro. Utilizou-se o meio MS, acrescido de 30g.L-1 de sacarose, 100mg.L-1 de mio-inositol e 6g.L-1 de agar, sendo o pH ajustado para 5,8. Os tratamentos consistiram da aplicacao de BAP e TDZ (5mM), cada um combinado com acido naftaleno-acetico (ANA), acido indolacetico (AIA) e acido indolbutirico (AIB) nas concentracoes de 5 e 10mM, com tres repeticoes de cinco explantes, num delineamento em blocos ao acaso. Inicialmente, os explantes foram submetidos a tres semanas de escuro, seguindo-se em ambiente a 25 ± 20C, luminosidade de 1500 – 2000 lux e fotoperiodo de 16 horas. A partir da saida dos explantes da condicao de escuro foram avaliadas semanalmente as percentagens de calos formados e calos regenerativos, assim como o numero de brotacoes e gemas. Houve formacao de calos em todos os tratamentos, sendo a presenca de BAP e TDZ importantes para formacao de calos regenerativos. Para as demais variaveis, os melhores tratamentos foram: BAP, quando se obteve 10 brotos/explante ou 39 gemas/explante; e BAP + AIB (5mM), que originou, em media, 5,6 brotos/explante ou 20 gemas/explante. As brotacoes originadas nos tratamentos com TDZ foram atrofiadas e vitrificadas.
File Format	PDF HTM / HTML
DOI	10.18539/cast.v5i3.289
Volume Number	5
Alternate Webpage(s)	https://periodicos.ufpel.edu.br/ojs2/index.php/CAST/article/download/289/284
Alternate Webpage(s)	https://doi.org/10.18539/cast.v5i3.289
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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