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Smooth Muscle Cell Phenotype Modulation and Contraction on Native and Cross-Linked Polyelectrolyte Multilayers
| Content Provider | Semantic Scholar |
|---|---|
| Author | Moussallem, Maroun D. Olenych, Scott G. Scott, Shannon Louise. Keller, Thomas C. S. Schlenoff, Joseph B. |
| Copyright Year | 2009 |
| Abstract | Smooth muscle cells convert between a motile, proliferative "synthetic" phenotype and a sessile, "contractile" phenotype. The ability to manipulate the phenotype of aortic smooth muscle cells with thin biocompatible polyelectrolyte multilayers (PEMUs) with common surface chemical characteristics but varying stiffness was investigated. The stiffness of (PAH/PAA) PEMUs was varied by heating to form covalent amide bond cross-links between the layers. Atomic force microscopy (AFM) showed that cross-linked PEMUs were thinner than those that were not cross-linked. AFM nanoindentation demonstrated that the Young's modulus ranged from 6 MPa for hydrated native PEMUs to more than 8 GPa for maximally cross-linked PEMUs. Rat aortic A7r5 smooth muscle cells cultured on native PEMUs exhibited morphology and motility of synthetic cells and expression of the synthetic phenotype markers vimentin, tropomyosin 4, and nonmuscle myosin heavy chain IIB (nmMHCIIB). In comparison, cells cultured on maximally cross-linked PEMUs exhibited the phenotype markers calponin, smooth muscle myosin heavy chain (smMHC), myocardin, transgelin, and smooth muscle alpha-actin (smActin) that are characteristic of the smooth muscle "contractile" phenotype. Consistent with those cells being "contractile", A7r5 cells grown on cross-linked PEMUs produced contractile force when stimulated with a Ca(2+) ionophore. |
| Starting Page | 3062 |
| Ending Page | 3068 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 19817347v1 |
| Alternate Webpage(s) | https://doi.org/10.1021/bm9007309 |
| DOI | 10.1021/bm9007309 |
| Journal | Biomacromolecules |
| Volume Number | 10 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 3-Mercaptopropionic Acid Artificial Cells Calcium Covalent Interaction Cultured Cells Elastic Modulus Genus Paa Greater Than Heating Ionophores MYH11 gene Microscopy, Atomic Force Muscle Cells Myocytes, Smooth Muscle Myosin Heavy Chains SLC3A2 gene Smooth muscle (tissue) Stiffness TPM4 gene Tropomyosin anatomical layer calponin polyelectrolyte 60 ammonium salt |
| Content Type | Text |
| Resource Type | Article |
