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Differentiation of pluripotent embryonic stem cells into the neuronal lineage in vitro gives rise to mature inhibitory and excitatory neurons
| Content Provider | Semantic Scholar |
|---|---|
| Author | Strübing, C. Ahnert-Hilger, Gudrun Shan, Jin Wiedenmann, Bertram Hescheler, Jürgen Wobus, Anna M. |
| Copyright Year | 1995 |
| Abstract | Embryonic stem (ES) cells represent a suitable model to analyze cell differentiation processes in vitro. Here, we report that pluripotent ES cells of the line BLC 6 differentiate in vitro into neuronal cells possessing the complex electrophysiological and immunocytochemical properties of postmitotic nerve cells. In the course of differentiation BLC 6-derived neurons differentially express voltage-dependent (K+, Na+, Ca2+) and receptor-operated (GABAA, glycine, AMPA, NMDA receptors) ionic channels. They generate fast Na(+)-driven action potentials and are functionally coupled by inhibitory (GABAergic) and excitatory (glutamatergic) synapses as revealed by measurements of postsynaptic currents. Moreover, BLC 6-derived neurons express neuron-specific cytoskeletal, cell adhesion and synaptic vesicle proteins and exhibit a Ca(2+)-dependent GABA secretion. Thus, the ES cell model enables the investigation of cell lineage determination and signaling mechanisms in the developing nervous system from a pluripotential stem cell to a differentiated postmitotic neuron. The in vitro differentiation of neurons from ES cells may be an excellent approach to study by targeted gene disruption a variety of neuronal functions. |
| Starting Page | 275 |
| Ending Page | 287 |
| Page Count | 13 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0925-4773(95)00446-8 |
| PubMed reference number | 8562428 |
| Journal | Medline |
| Volume Number | 53 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/0925477395004468 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/0925477395004468 |
| Alternate Webpage(s) | https://doi.org/10.1016/0925-4773%2895%2900446-8 |
| Journal | Mechanisms of Development |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
